Ronald N Harty
Associate Professor of Microbiology (with tenure)Contact Information
Rosenthal Building Room 412
Philadelphia , PA 19104-6049
Office: 215 573-4485
Fax: 215 898-7887Email:email@example.com
BS (Biology) University of Lowell, Massachusetts, 1985
Ph.D. (Microbiology/Virology) Lousiana State University Medical Center, 1991
PublicationsSearch PubMed for Articles
Description of Research Expertise
1. Molecular Mechanisms of Negative-Sense RNA Virus Assembly/Budding. Viruses
studied include: Filoviruses (Ebola and Marburg) and Rhabdoviruses (Vesicular
Stomatitis and Rabies).
2. Virus-Host Interactions that Modulate Filovirus and Rhabdovirus Assembly/Budding.
3. Innate Immune Defenses against Filoviruses and Rhabdoviruses.
4. Use of VSV Recombinants and FLIM to study Herpes Simplex Virus Type 1 (HSV-1)
Glycoprotein B mediated Virus-Cell Fusion.
Key words: Ebola, Marburg, VSV, Matrix Proteins, VP40, Budding, VLPs, Reverse-Genetics, Virus-Host Interactions, Innate Immunity.
Description of Research
My laboratory is focused mainly on the molecular events that lead to virus assembly and budding. Our model virus systems include vesicular stomatitis virus (a rhabdovirus that causes disease in bovine and equine species), and the filoviruses Ebola and Marburg (emerging, zoonotic pathogens and potential agents of bioterrorism). Our studies are focused on the viral matrix proteins which are the major building blocks of the virus and function to direct virion assembly and budding. We are particularly interested in understanding how these viral matrix proteins interact with host proteins to facilitate the budding process. We are also interested in the host innate immune response to virus infection, and identifying antivirals that can inhibit egress and spread of filoviruses and rhabdoviruses. A second area of study is to utilize reverse-genetics to generate and recover VSV recombinants expressing the surface gB protein of HSV-1 for use in studies to dissect the structure and function of gB during virus-cell fusion. My lab utilizes a wide-array of techniques for these studies such as, virus-like particle (VLP) budding assays, reverse-genetics to generate VSV recombinants, bimolecular complementation, ubiquitination and ISGylation assays, and fluorescence lifetime imaging microscopy (FLIM).
Dr. Yuliang Liu – Senior Postdoctoral Fellow
Dr. Yonggang Qu – Visiting Scientist.
Ms. Sasha Stone – Penn Undergraduate Bio-Engineering Major (Independent Study)
Mr. Benoit Clerc-Renaud – 2011 NIH/Merial Veterinary Scholar
Liu, Y., Stone, S., and Harty, R. N. : Characterization of filovirus protein-protein interactions in mammalian cells using bimolecular complementation. J. Infect. Dis in press.
Okumura, A. and Harty, R. N. : Rabies Virus Assembly and Budding. In: Research Advances in Rabies (Alan Jackson, Ed.) Advances in Virus Research 79: 23-32, Elsevier, 2011.
Liu, Y., Cocka, L., Okumura, A., Zhang, Y.A., Sunyer, J. O., and Harty, R. N.: Conserved motifs within Ebola and Marburg virus VP40 proteins are important for stability, localization, and subsequent budding of virus-like particles. J. Virol. 84(5): 2294-2303, 2010.
Okumura, A., Pitha, P. M., Yoshimura, A., and Harty, R. N. : Interaction between Ebola virus glycoprotein and host toll-like receptor 4 leads to induction of proinflammatory cytokines and SOCS1. J. Virol. 84(1): 27-33, 2010.
Liu, Y. and Harty, R. N. : Viral and Host Proteins that Modulate Filovirus Budding. Future Virology 5(4): 481-491, 2010.
Harty, R. N.: No Exit: Targeting the Budding Process to Inhibit Filovirus Replication. Antiviral Research 81(6) 2009.
Harty, R. N., Pitha, P. M., and Okumura, A. : Antiviral Activity of Innate Immune Protein ISG15. J. Innate Immun. J. Innate Immun. 1(5) 2009.
Okumura, Atsushi. Pitha, Paula M. Harty, Ronald N.: ISG15 inhibits Ebola VP40 VLP budding in an L-domain-dependent manner by blocking Nedd4 ligase activity. Proceedings of the National Academy of Sciences of the United States of America 105(10): 3974-9, Mar 11 2008.
Wirblich, C., Tan, G. S., Papaneri1, A., Godlewski, P. J., Harty, R. N.*, and Schnell, M. J.* : PPEY motif within the rabies virus matrix protein is essential for efficient virion release and RV pathogenicity, but not immunogenicity. J. Virol. 82(19): 9730-8, 2008.
Johnson, Reed F. McCarthy, Sarah E. Godlewski, Peter J. Harty, Ronald N.: Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles. Journal of Virology 80(11): 5135-44, Jun 2006.
Irie, Takashi. Licata, Jillian M. Harty, Ronald N.: Functional characterization of Ebola virus L-domains using VSV recombinants. Virology 336(2): 291-8, Jun 5 2005.
Licata JM, Johnson RF, Han Z, Harty RN: Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles J Virol. 78(14): 7344-51, Jul 2004.