Loading

Reference Andrology Laboratory

 The Reference Andrology Laboratory provides complete testing of neat, cooled and frozen-thawed semen from mammalian and avian species. The primary purpose of these services is to aid practitioners in their differential diagnosis of individual/herd/flock reproductive problems.

These services are also frequently used by practitioners and studs as a third-party quality control component in an ongoing stud auditing process.

The laboratory strives to perform objective, validated techniques for assessing samples for the basic spermiogram parameters of sample volume, motility, morphology, and concentration. With advanced notification, we will also try to accommodate requests for supplemental assessment techniques on sperm subcellular structures. We also offer semen extender analysis and microbiological testing of the extended semen product and purified water used in extenders.

Routine Semen Analyses:

  • Acrosome morphology
  • Sample osmolarity
  • Sample conductivity, pH
  • Sample Volume
  • Sperm motility
  • Sperm viability
  • Sperm morphology
  • Sperm Concentration
  • Total sperm number/dose
  • Various flow cytometry parameters

 

Routine Microbiology Evaluation*, including:

  • Direct, aerobic culture / Isolation
  • Enrichment techniques, if needed
  • Genus species identification of isolates
  • Antimicrobial susceptibility testing  (per organism)
 

Other Services:

  •  On-site auditing of stud processes and techniques
  • Semen extender analyses
  • Spermicidal assessment of materials and isolates
  • Water analysis

 

QC Analysis Graphs

– 2014 –

Quarter 1

14Q1 Conc

14Q1 Morph 

14Q1 Mot 

 

– 2013 –

Quarter 4

Quarter 3

Quarter 2

 

Quarter 1

       

 

 – 2012 –

 Quarter 4

Quarter 3

 

 

 

Quarter 2

 

Quarter 1

     

  – 2011 –

Quarter 4

Quarter 3

Quarter 2

Quarter 1

 

--earlier QC graphs available upon request--

  

 

GC Althouse, DVM, MS, PhD
Diplomate, American College of Theriogenologists
Member, American Association of Swine Veterinarians
Member, American Society of Andrology
Member, American Veterinary Medical Association
Member, Society for Theriogenology

Von Miller-Lux, BS, Lab Manager
Member, American Association of Bioanalysts
ymiller@vet.upenn.edu

Betty Osborne, BS, MT (ASCP), Lab Technician
Member, American Society for Clinical Pathology
bettyo@vet.upenn.edu

Cheryl O'Sullivan, BA, MA, Admin. Coordinator, Reports, Web site, Billing
cherylo@vet.upenn.edu