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| Qualifications |
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1987 – 1993 Ph.D., Cell Biology, Institute for Protein Research, Moscow, Russia |
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| Memberships |
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ASCB |
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| Research Interests |
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The goal of our research is to investigate the physiological role of a previously uncharacterized posttranslational modification, protein arginylation. Knockout of the enzyme responsible for arginylation, ATE1, results embryonic lethality in mice and multiple defects related to heart development and blood vessel remodeling (angiogenesis). Our recent work showed that arginylation regulates many proteins involved in cytoskeleton, cell motility, signaling, and metabolism, and uncovered some mechanisms of this regulation. Our current studies are focused on three major directions: (1) identification of the ATE1 protein targets and studying the effect of arginylation on their properties and functions; (2) studies of the structure and molecular properties of the mouse ATE1 enzymes; and (3) discovering the mechanisms and pathways that lead to the global physiological effects of protein arginylation. |
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| Selected Publications |
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Abstract and Full Text Karakozova M, Kozak, M, Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., and Kashina, A. (2006) Arginylation of Beta Actin Regulates Actin Cytoskeleton and Cell Motility. Science. On line: June 22, 2006 Rai R, Mushegian A, Makarova K, Kashina A. (2006) Molecular dissection of arginyltransferases guided by similarity to bacterial peptidoglycan synthases. EMBO Rep. On line: July 7, 2006 R. Rai and A. Kashina (2005) Identification of mammalian arginine transferases that modify a specific subset of protein substrates. Proc. Natl. Acad. Sci., USA 102:10123-10128 I. Sorokina and A. Kashina (2005) Archived gels as a tool for identification of protein complexes: POLO kinase cofractionates with Drosophila 205 kDa MAP and ncd in mitotic embryonic extracts. Analytical Biochemistry 344:55-157 A. Kashina and V. Rodionov (2005) Intracellular Organelle Transport: Few Motors, Many Signals. Trends in Cell Biology 15:396-398 Y.T. Kwon, A.S. Kashina, (equal contribution), I.V. Davydov, R.G. Hu, J. Y. An, J.W. Seo, F. Du, and A. Varshavsky (2002) An essential role of N-terminal arginylation in cardiovascular development. Science, 297(5578):96 Y. T. Kwon, A.S. Kashina (equal contribution), and A. Varshavsky (1999) Alternative Splicing Results in Differential Expression, Activity and Localization of the Two Forms of Arginyl-tRNA-Protein Transferase, a Component of the N-End Rule Pathway. Mol. Cell. Biol., 19:182-193 A.S. Kashina, G.C. Rogers, and J.M.Scholey (1997) The bimC subfamily of kinesins: essential bipolar mitotic motors driving centrosome separation. Review.Biochim. Biophys. Acta, 1357:257-271. A.S. Kashina, J.M.Scholey, J.D.Leszyk, and W.M.Saxton. (1996) An essential bipolar mitotic motor. Nature 384:225 A.S. Kashina, R.J.Baskin, D.G.Cole, K.P.Wedaman, W.M.Saxton, and J.M.Scholey (1996) A bipolar kinesin. Nature 379:270-272 |
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