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New Bolton Center Kennett Square, PA
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  • Beiting Laboratory

    We study the biological basis of diseases caused by microbes -- whether it be a parasitic worm, a pathogenic bacterium, or a complex microbial community in the gut. Our group makes up the Center for Host-Microbial Interactions, at Penn Vet, and our research leverages a diverse skill set that cuts across the disciplines of genomics, microbiology and immunology. We engage in collaborative projects that benefit from close interactions with veterinarians and human clinicians alike. Our research embodies the idea of 'One-Health' - that humans, animals and the environment are interconnected, and that we all live in a microbial world. We are located at the The University of Pennsylvania, in The Department of Pathobiology at the School of Veterinary Medicine.

    Read More About The Beiting Laboratory
  • Striepen Laboratory

    Welcome to the Striepen lab

    We study the cell and molecular biology of parasites, and use our findings to develop new treatments. Most of our research is focused on Cryptosporidium and Toxoplasma, two protozoan parasites that threaten small children and those with weakened immune systems.

    Read More About The Striepen Laboratory
  • Havemeyer Equine Behavior Research Lab

    Semi-feral pony herd at New Bolton Center

    The Equine Behavior Program and Laboratory at New Bolton Center has grown from within the Section of Reproductive Studies.  Since the early 1980s the program, has had research as its core activity.  The program has included involvement in related clinical and teaching in the veterinary school and continuing education programs nationally and internationally.  The initial research focus of the laboratory was on stallion reproductive physiology and behavior. 

    Early research concentrated on the physiology and pharmacology of libido, erection, and ejaculation, with immediate application to clinical problems in breeding stallions and with relevance to the understanding of human sexual dysfunction. 

    Another long-standing research interest of our laboratory has been the effects of experience on sexuHavemeyer Barnal function.  In the 1990s our research and clinical work expanded beyond stallions to include reproductive and general behavior problems of horses.

    The Behavior Lab is housed in The Havemeyer Barn at The Georgia and Philip Hofmann Center for Animal Reproduction. 

    Read More About The Havemeyer Equine Behavior Research Lab
  • Ortved Laboratory

    The Ortved Laboratory at New Bolton Center is focused on understanding the pathophysiology of post-traumatic osteoarthritis (PTOA) and developing gene and cell-based therapies to help regenerate cartilage and prevent the development of PTOA following joint injury.

    Due to the many similarities in joint biomechanics and propensity for PTOA, our lab uses the horse as a large animal model for human joint disease. Our goal is to develop translational regenerative therapies that would benefit both the equine and human patient.

    Lab Mission

    • To improve cartilage repair using stem cell and gene therapy.
    • To limit the long-term effects of joint trauma through gene therapy immunomodulation of the joint.
    • To further elucidate the pathogenesis of post-traumatic osteoarthritis (PTOA).

    Dr. Kyla Ortved, New Bolton Center

    Interested in Working With Us?

    We are always seeking highly motivated students and post-doctoral fellows with an interest in:

    • Orthopedic research
    • Joint disease
    • Stem cell therapy
    • Gene therapy
    • Regenerative medicine
    • Cartilage biology

    Contact: Dr. Kyla Ortved at kortved@vet.upenn.edu

    Read More About The Ortved Laboratory
  • Kubin Laboratory

    Laboratory of Neurochemical Mechanisms of REM Sleep and Sleep-Related Respiratory Disorders

    We are interested in neural mechanisms of cardiorespiratory disorders during sleep associated with a common disorder known as the Obstructive Sleep Apnea (OSA) Syndrome. We also explore the basic mechanisms responsible for the generation of Rapid Eye Movement (REM) stage of sleep. Research is led by Dr. Leszek Kubin.

    Read More About The Kubin Laboratory
  • Immune Regulation Laboratory

    Professor Oliver Garden’s laboratory, historically based at the Camden Campus of the Royal Veterinary College and now at the School of Veterinary Medicine at the University of Pennsylvania, Philadelphia, specializes in cellular immunology, with a specific expertise in regulatory T cell (Treg) and myeloid-derived suppressor cell (MDSC) biology. Oliver started his laboratory with the aid of a Wellcome Trust Advanced Fellowship in 2001 within the Department of Immunology, Imperial College London, Hammersmith Campus, following post-doctoral training at the University of South Carolina School of Medicine and a Residency in Small Animal Internal Medicine at Cornell University College of Veterinary Medicine.

    Immune-Regulation-Lab-research-themes-no-USP 475

    Read More About The Immune Regulation Laboratory
  • Wolfe Laboratory

    Welcome to the Wolfe Lab, supervised by John H. Wolfe, Professor of Pathology and Medical Genetics in Pediatrics and Director, Walter Flato Goodman Center for Comparative Medical Genetics.

     Description of Research 

    Our lab works on transferring disease correcting genes into the central nervous system (CNS) in animal models of human genetic diseases. In these diseases, the CNS is often not rescued by the therapies that help the rest of the body. The lab studies both ex vivo gene transfer into neural stem cells that are then transplanted and in vivo transfer using vectors injected directly into the brain.

    Our studies involve comparisons of both the vectors used to introduce the genes into cells and the properties of the genes themselves. Additionally, we examine the ability of different cell types and subregions of the brain to be corrected. We also pursue new methods to follow the corrected cells and the expression of the correcting gene in the live animal using MRI and PET techniques. For a complete understanding of the therapy, we also are working on achieving a better understanding of the mechanism of these diseases in the brain.

    Projects

    Projects involve the molecular design and engineering of vectors, the understanding of the fate of vector-transferred genes in the brain, the regulation of gene expression from vectors, the biology of neural stem cell, the study of induced pluripotent stem cells (iPS), the use of imaging studies in genetic disease and gene therapy, and the proteomic and genomics analysis of the neurodegenerative brain. For students, projects can be tailored to interests, learning goals, and experience.

    Read More About The Wolfe Laboratory
  • Povelones Laboratory

    Research Interests

    pove-lab-logo-2

    Our main research interest is innate immune recognition and elimination of pathogens. Our work focuses on the interaction between mosquitoes and the animal and human pathogens they transmit. As the most species-rich group of animals on the planet occupying a vast array of ecological niches, insects are a fantastic example of the potency of innate defenses.

    PoveLab in the news:
    ★ News piece by Tom Avril in Philly.com
    ★ TV spot by John McDevitt for CBS 3 Philly
    ★ See us on Xploration Awesome Planet "Insects" (Season 3 Episode 10)
    ★ View UPenn Spotlight on Our Work

    Rather than passive or willing carriers of pathogenic organism, mosquitoes are actually amazing pathogen killers. Taking mosquito interactions with malaria parasites as an example, the vast majority of the parasites ingested when a mosquito bites a malarious person are attacked and eliminated before they can mount an infection in the mosquito. It is the few parasites that survive (even one is sufficient), that are ultimately responsible for disease transmission. Similar interactions occur between mosquitoes and the other pathogens they transmit, like canine heartworm (Dirofilaria) and arboviruses (Zika, Dengue, Yellow Fever, West Nile, and Chikungunya).

    Arthropod vectors such as mosquitoes, sand flies and ticks are responsible for transmission of a large number of animal and human diseases worldwide. Studying these organisms may reveal general insights about innate immune defense mechanisms as well as provide novel avenues for controlling the terrible diseases they spread.

    Some of the questions we are addressing:

    • What is the basis of pathogen recognition by the mosquito innate immune system and how do some pathogens manage to escape?
    • What is the biochemical mechanism leading from innate recognition to pathogen killing?
    • How is mosquito complement regulated?
    • How does steroid hormone signaling regulate mosquito immunity?
    Read More About The Povelones Laboratory
  • Lengner Laboratory

    Our lab is broadly interested in the mechanisms by which stem cells acquire and maintain developmental potency. We are also exploring how deregulation of these mechanisms can contribute to oncogenic transformation, tumorigenesis, and tissue regeneration in response to injury, while looking forward to learn how we might manipulate these mechanisms for application in disease modeling and regenerative medicine.
     
    In the mammalian soma, tissue-specific stem cells capable of maintaining the proliferative output necessary for tissue organization and function exist in a state Lengner image 6 copyof multipotency (the ability to generate any cell type of that particular tissue, in contrast to the pluripotent state embodied by embryonic stem cells capable of generating all cell types of the mammalian organism). In highly proliferative tissues such as the epithelial lining of the intestine, data from our lab and others has begun to establish a model in the stem cell compartment is organized into a hierarchy, with a mostly dormant population of long-live, radio-resistant reserve stem cells at the top of this hierarchy. When activated, these reserve stem cells give rise to a second, highly proliferative, radiosensitive short-term stem cell that bears the daily proliferative burden required to maintain tissue homeostasis.  
     
    Our lab is focused on understanding the relationship between these two stem cell populations, the molecular determinants of reserve intestinal stem cell activation, and how deregulation of the reserve intestinal stem cell compartment contributes to disease states such as colorectal cancer or acute gastrointestinal radiation injury.
     
    We have recently identified the Msi family of RNA binding proteins as potent oncoproteins in both hematopoietic and intestinal malignancies. Msi proteins are expressed in putative somatic Lengner image 8 copystem cell compartments, are frequently found to be overexpressed in advanced cancers, and are known to govern asymmetric cell division in Drosophila melanogaster (a process thought to maintain the somatic stem cell niche in mammals). Using mouse genetic approaches integrated with human patient data, we have recently demonstrated that MSI2 acts as an intestinal oncogene, driving activation of the mTORC1 complex and uncontrolled stem cell expansion. We are currently pursuing the role of Msi proteins in epithelial stem cell compartments using tissue-specific gene ablation and drug-inducible gene activation. The effects of Msi proteins on stem cell maintenance and oncogenic transformation are being tied to their RNA binding capacity using CLIP-Seq analysis (immunoprecipitation of Msi-interacting RNAs followed by massively parallel sequencing) in order to determine how specific Msi-RNA interactions affect stem cell self-renewal and oncogenic transformation.
     
    While murine genetic systems are the primary tool of the laboratory, we also work to model human genetic gastrointestinal disorders using induced pluripotent stem (iPS) cells generated from patients. Generation of isogenic diseased and disease-allele corrected iPS cell lines using nuclease-mediated homologous recombination followed by directed differentiation into intestinal tissue provides a controlled platform not only for studying the molecular mechanisms underlying  

    Photos above: Label retaining cells of the intestinal crypts are identified by loading all cells with a Histone H2B protein fused to a green fluorescent protein (left). Several weeks later, only cells that do not divide retain the fluorescent label in their chromatin.

    A glimpse of the rare reserve intestinal stem cell (red).   This cell is capable of regenerating the entire intestinal lining after injury such as exposure to high doses of radiation.
    Read More About The Lengner Laboratory
  • Laminitis Laboratory at New Bolton Center

    Laminitis is a common and debilitating disease that affects the folded and interdigitating tissues, called the lamellae, which connect the hoof wall to the underlying tissues of the horse’s foot. The lamellae normally allow the transfer of the horse's weight from the skeletal elements of the digit to the hoof wall.

    Dr. Galantino-Homer founded the Laminitis Laboratory at New Bolton Center  in 2008. The Laminitis Laboratory was formed in part due to the tragic loss of the 2006 Kentucky Derby winner, Barbaro, to laminitis in January, 2007.

    Our goal is to better understand laminitis pathogenesis in order to improve the prevention, diagnosis, and treatment of this disease.

    Our studies include the investigation of laminitis pathogenesis using protein biochemistry and histopathological analysis, characterization of the epidermal stem cell population in the lamellae, identification of diagnostic serum biomarkers for laminitis, and establishing an in vitro culture system for equine hoof epidermal cells to minimize the use of live horses for laminitis research.

    The Laminitis Laboratory is home to the Laminitis Discovery Database, an archive of pathology images, histology slides, and frozen lamellar tissue and serum from naturally occurring cases of laminitis and unaffected control horses. These materials are being used for several published and ongoing multi-institutional collaborative studies.

    Read More About The Laminitis Laboratory at New Bolton Center
  • Sunyer Laboratory

    Sunyer nature fig 1 The studies of Dr. Sunyer's lab focus on basic and applied aspects of the fish immune system. Moreover, as teleost fish represent the most ancient living bony species with an immunoglobulin-based adaptive immune system, we use these species to study key aspects of the evolution of sunyer JLB fig 2adaptive immunity. Our main animal model is Rainbow trout.  While earlier work focused on investigating the structure, function and evolution of fish complement  (see below refs# 1-7), during the last 7 years our studies have mainly focused on B cells and mucosal immunity aspects of teleost fish.

    Read More About The Sunyer Laboratory
  • Center for Host-Microbial Interactions

     Center for Host Microbe InteractionsThe Penn Vet Center for Host-Microbial Interactions (CHMI) formed in 2013 as an interdisciplinary center that helps faculty leverage cutting-edge genomic approaches to understand how microbes (viruses, bacteria and parasites) influence animal health and disease. These so-called ‘host-microbial interactions’ represent an ongoing evolutionary arms-race between mammals and the microbial world we live in. 

    Most people are familiar with well-known viral infections caused by influenza, ebola; or bacterial infections caused by Salmonella or E. coli.  In each case, these pathogens can spread from animals to people, highlighting the notion that humans, animals, and our environment are inextricably connected by infectious diseases — a concept termed ‘One-Health’. 

    In the past few years it has become increasingly clear that just as there are microbes that cause disease, there are also beneficial microbes that are crucial in maintaining health.  Beneficial bacteria colonize our gut, skin and urogenital tract at birth and these complex microbial communities - termed a microbiome - develop just as our organ systems develop.  

    Researchers at Penn Vet, with assistance from CHMI, are actively studying the role of these the microbiome in animal diseases ranging from atopic dermatitis, inflammatory bowel disease, to mental health.

    Our mission is to better understand and treat disease through the study of microbes and the diverse ways animals respond to viruses, bacteria and parasites.

    Our Goals:

    • Establish an internationally recognized center that is the first of its kind at veterinary schools
    • Engage the broader Penn Vet community in host-microbial research that leverages ‘omic approaches
    • Develop stronger ties across schools at UPenn
    • Leverage spontaneous animal models of disease commonly seen at the Penn Vet Ryan Hospital
    • Establish a convenient ‘in-house’ solution for Penn Vet labs to analyze complex data sets that result from systematic studies of gene expression, microbial whole-genome sequencing, and the composition of microbial communities living on animals.
    Read More About The Center for Host-Microbial Interactions
  • Dou Laboratory

    Integrated System Approach for Nutrient Management

    Nitrogen and phosphorus are essential nutrients for growing plants and animals. Insufficient nutrients diminish yields; excessive nutrients translate to wasted resources and environmental pollution. The work of Dr. Dou’s group features an integrated system nutrient management approach, coupling nutrient optimization in animal feeding with manure management and targeted nutrient application to crops for enhanced production efficiency and reduced environmental footprint.

    • Please review relevant publications in 'Publications' tab below.

    Integrated whole farm management based on nutrients (pathogen) flow pathway

     

    Whole Farm Management 

    Pathogens, Antimicrobials, and the Environmental Fate

    Food animals are important reservoirs of zoonotic pathogens as well as antimicrobial resistant genes.  What happens to these “microbial pollutants” in the post-shed environment? How long do they survive under different management conditions? What is their distribution pattern in the intrinsically linked farming sectors and the dissemination pathway? The research of Dr. Dou’s group addresses some of these critical issues.

    • Please review relevant publications in 'Publications' tab below.

    Food Security and Sustainability

    Global food security and sustainability is an issue that intersects nearly all of the contemporary challenges the world is struggling to deal with today, e.g. water shortage, energy crisis, resource inefficiency, biodiversity decline, inequality and hunger and poverty.  Dr. Dou collaborates with national and international experts to examine this topic from different perspectives.

    • Please review relevant publications in 'Publications' tab below.

    Latest News

    The Last Food Mile Conference is being held at the University of Pennsylvania on December 8-9, 2014:

    This conference will bring together experts from national and international, academic and industrial, public and private sectors, to discuss:

    • Where food losses occur along the food supply chain, why, and how much
    • What food waste reduction measures work effectively, lessons learned and barriers encountered
    • What policies and interventions are critically needed for moving forward
    Read More About The Dou Laboratory
  • Wang Laboratory

    Our group focuses on the study of regulation of meiosis and the biology of small non-coding RNAs - piRNAs in mice and humans.

    Meiosis, a cell division unique to germ cells, allows the reciprocal exchange of genetic material between paternal and maternal genomes. Meiosis generates the genetic diversity necessary for evolution of species.

    Abnormality in meiosis is a leading cause of birth defects and infertility. Our research interests include molecular genetics of chromosomal synapsis, DNA double-strand break repair, homologous recombination, genetic causes of male infertility in humans, piRNA biogenesis, and epigenetic silencing of transposable elements.

    We have performed two genome-wide screens to identify novel factors that regulate germ cell development in mice: a genomics screen has identified 36 germ cell-specific genes; a proteomics screen has uncovered more than 50 meiotic chromatin-associated proteins.

    Functional characterization of a number of new genes in our laboratory has uncovered novel regulatory mechanisms underlying key biological processes unique to germ cells. On one hand, our studies provide molecular insights into the development of germ cells in mice. On the other hand, these mouse studies have important implications for understanding the genetic causes of male infertility in humans.

    We employ a battery of the state-of-the-art technologies in our research: gene targeting, genome editing, genomics, proteomics, cell biological and molecular biological approaches.

    Read More About The Wang Laboratory
  • Field Office Lancaster County-PADLS

    PADLS Field Office Lancaster CountyLocated in the northwest part of Lancaster County, the Field Office is a convenient location for veterinarians, farmers, and wildlife rehabilitators living in the western part of the county. The Field Office provides services in both avian pathology and aquaculture including necropsies of birds and fish and sample drop-off for microbiological testing, serology, and toxicology. Clinical and environmental samples received at the Field Office are taken to New Bolton Center for testing.

    As part of PADLS, the Field Office supports PADLS’ praquaculture285ogram objectives and mission. The combined resources and technical expertise of leading scientists at PADLS laboratories provide state-of-the-art diagnostic technology supporting veterinarians’ efforts to advance animal and public health initiatives and support producers in assuring a safe, secure, and abundant food supply.

    Read More About The Field Office Lancaster County-PADLS
  • Aquaculture-PADLS

    PADLS AquacultureThe Aquaculture Laboratory provides necropsy and diagnostic testing of commercial and ornamental fish. With facilities at both NBC and the Manheim Field Office, the Aquaculture Laboratory is easily accessible for live fish drop-off.  The laboratory provides testing for diagnostics purposes only. Any regulatory testing should be submitted through the Pennsylvania Veterinary Laboratory. More information can be found on the PADLS website.

    As part of PADLS, the Aquaculture Laboratory supports PADLS’ program objectives and mission. The combined resources and technical expertise of leading scientists at PADLS laboratories provide state-of-the-art diagnostic technology supporting veterinarians’ efforts to advance animal and public health initiatives and support producers in assuring a safe, secure, and abundant food supply.

    Read More About The Aquaculture-PADLS
  • Toxicology Laboratory-PADLS

    PADLS ToxicologyLocated in the Myrin Building on the New Bolton Campus, the toxicology laboratory is equipped with the latest technology and a knowledgeable support staff to provide full veterinary diagnostic toxicology services. Animals can be exposed to toxic chemicals in a number of ways: ingestion of contaminated feed or foreign objects, inhalation, or skin contact. Through identification of such toxic chemicals, the toxicology laboratory helps to diagnose and prevent toxicosis in agricultural animals, pets, and wildlife. In addition to toxins, the laboratory has the ability to identify the levels of nutritionally relevant compounds including metals and vitamin E in animal samples.

    As part of PADLS, the toxicology lab supports PADLS' program objectives and mission. The combined resources and technical expertise of leading scientists at PADLS laboratories provide state-of-the-art diagnostic technology supporting veterinarians’ efforts to advance animal and public health initiatives and support producers in assuring a safe, secure, and abundant food supply.

    Read More About The Toxicology Laboratory-PADLS
  • Avian Pathology Laboratory-PADLS

    Avian Pathology smallThe Avian Pathology service strives to provide the best possible diagnostic service to Pennsylvania and regional producers, hatcheries, service personnel, feed companies, and breeder companies. Over the past decade, the service has expanded to include pet bird cases submitted by regional veterinarian, pigeons, waterfowl and birds of prey cases submitted by local wildlife organizations, backyard poultry flocks, and exhibition/show birds. Services include avian necropsies, serologic testing, virus isolation, and PCR. The board certified veterinary staff is not only qualified to provide accurate diagnostics but also advise on the treatment, control, and eradication of infectious diseases. Avian field investigation also adds to the array of services available.

    As part of PADLS, the Avian Pathology service supports PADLS’ program objectives and mission. The combined resources and technical expertise of leading scientists at PADLS laboratories provide state-of-the-art diagnostic technology supporting veterinarians’ efforts to advance animal and public health initiatives and support producers in assuring a safe, secure, and abundant food supply.

    Read More About The Avian Pathology Laboratory-PADLS
  • Large Animal Pathology Laboratory-PADLS

    The Large Animal Pathology Service provides complete necropsy and biopsy services to the patients of New Bolton and referrals from veterinarians throughout the state.

    Immunohistochemistry adds a special dimension to Widener Hospital’s diagnostic service and to the work of Penn researchers. This special staining technique uses color-labeled antibodies to identify infectious agents and special cell compLarge Animal Pathologyonents in histology sections. 

    As part of PADLS, the Large Animal Pathology Service supports PADLS’ program objectives and mission.

    The combined resources and technical expertise of leading scientists at PADLS laboratories provide state-of-the-art diagnostic technology supporting veterinarians’ efforts to advance animal and public health initiatives and support producers in assuring a safe, secure, and abundant food supply.

    Read More About The Large Animal Pathology Laboratory-PADLS
  • Microbiology Laboratory-PADLS

    PADLS MicrobiologyThe microbiology laboratory is separated into three subgroups: Clinical Microbiology, Molecular Microbiology, and PEQAP.

    Located in the Myrin Building on the New Bolton Campus, the Clinical Microbiology laboratory is equipped with the latest technology and a knowledgeable support staff to provide diagnostic microbiology testing. Consultation and field investigation are also available. Testing includes aerobic, anaerobic, and fungal culture, and antibiotic susceptibility testing. 

    Molecular Microbiology uses sophisticated techniques to isolate and identify viruses and bacteria including polymerase chain reaction (PCR) and real-time reverse transcriptase-PCR. These methods are commonly used to identify Avian Influenza, Salmonella spp., and Streptococcus equi (Strangles).

    In addition to standard microbiological testing, the microbiology laboratory also performs egg testing as part of the Pennsylvania Egg Quality Assurance Program (PEQAP) and the FDA Egg Safety Rule. Farms that participate in these programs demonstrate their concern about food safety and produce a quality egg which helps to assure consumer confidence in eggs.

    As part of PADLS, the microbiology laboratory supports PADLS' program objectives and mission. The combined resources and technical expertise of leading scientists at PADLS laboratories provide state-of-the-art diagnostic technology supporting veterinarians’ efforts to advance animal and public health initiatives and support producers in assuring a safe, secure, and abundant food supply. 

    Read More About The Microbiology Laboratory-PADLS