Watch the Miracle: the Foal's Ultrasounds & Videos

You can follow the miracle of Boone by watching these videos. Here, you'll see the initial ICSI (Intracytoplasmic Sperm Injection) technique, along with Boone's first ultrasounds. It's a rare and special view into the creation and gestation of an unborn foal.

ICSI: Intracytoplasmic Sperm Injection

Intracytoplasmic Sperm Injection

This video shows a laser gradually drilling a hole through the outer layer (the zona pellucida) of an equine egg. Once the zona pellucida is penetrated, a tiny needle is advanced through the hole in the zona, then through the egg's cell membrane and into the center of the egg.  A single sperm from a stallion is then gently injected and the needle is withdrawn.

Reproductive technology has been a strength of Penn Vet's since Dr. Ralph Brinster's groundbreaking research on mammalian embryo culture in the 1960s and transgenesis in the early 1980s. It is fitting that our clinical services continue in this tradition by offering advanced reproductive services for our patients. 

Meet Logan, one of our two newest Penn Vet Working Dogs:  https://scontent-b-iad.xx.fbcdn.net/hphotos-ash3/t1/1908181_609554829116774_830364700_n.jpg  and here's Quest, already too big for his britches.  https://scontent-b-iad.xx.fbcdn.net/hphotos-frc3/t1/1609974_609555639116693_1729526606_n.jpg  Both are 8 weeks old, and already working!!
After the ICSI procedure, the newly fertilized egg is cultured under very special conditions in a  laboratory incubator for about eight days, so the embryos will develop.  This picture shows two horse embryos four days after fertilization using ICSI.  After another four days in the incubator, these embryos will be ready to transfer to a surrogate mare.


Ultrasounds of the Foal
Foal ultrasound, My Special Girl, NBC
My Special Girl's pregnancy was created by transferring an eight-day old horse embryo into her uterus.  This ultrasonographic examination was performed on April 19, 2013, four days after the embryo was transferred.  The tiny black circle is My Special Girl's 12-day-old pregnancy, measuring nearly 3 mm.

Foal ultrasound, New Bolton Center
This ultrasonographic examination of My Special Girl's pregnancy was performed on January 27, 2014 (at around 9 months of gestation). The image shows the foal's eye, measuring 3.27 cm by 2.98 cm. If you look closely, the lens is even visible. Veterinarians can use the size of the foal's eye to help determine if the pregnancy is developing normally.  The measurements from My Special Girl's in utero foal are normal for a pregnancy at this stage of development.


Ultrasound Videos

Foal's eye during an ultrasound examination on January 27, 2014.

Foal's heartbeat during an ultrasound examination on January 27, 2014.