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New Bolton Center Kennett Square, PA
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610-444-5800
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Ryan Hospital Philadelphia, PA
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215-746-8911
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Mouse OXplus, Airway Physiology Core

Equipment


 
Airway Physiology Core Equipment
  • flexiVent®
    • (Comparable system to the Buxco® FinePointe Resistance/Compliance system)
  • The flexiVent® provides the user with the ability to assess the mechanical properties of the respiratory system.
  • Using clinically relevant aerosolized methacholine doses, lung function can be analyzed in murine models of genetic knockdown, disease, or damage. 
  • Buxco® FinePointe Resistance/Compliance system
    • (Comparable system to the flexiVent®) 
  • The Buxco® FinePointe Resistance/Compliance system provides the user with the ability to assess the mechanical properties of the respiratory system.
  • Using clinically relevant aerosolized methacholine doses, lung function can be analyzed in murine models of genetic knockdown, disease, or damage.

Airway Phys Core p1

  • Buxco® FinePointe Resistance/Compliance system-RC Compliance System (1/2)

  • The unit attached to the computer and FinePointe software delivers accurate doses of aerosolized methacholine via a nebulizer to an anesthetized mouse. The unit registers via forced inhalation any changes or obstructions to breathing as compared to baseline.

Airway phys core photo 3

  • The complete set up of the Buxco® FinePointe Resistance/Compliance system (2 units connected to one computer)
Airway Phys Core equip 2
  • This screen shows an example dataset that was generated from an experiment. Key data include lung Resistance (top left graph) and Total Volume of lung (Top right graph).  The former increases and the latter decreases as methacholine is administered in escalating doses.
  • Buxco® Small Animal Whole Body Plethysmography
  • Buxco® Small Animal Whole Body Plethysmography: Whole Body Plethysmography is a non-invasive way to measure total lung capacity and airway resistance. The system utilizes a chamber hooked up to a nebulizer and can detect changes airflow in the chamber in response to methacholine doses. This method requires no sedation and can potentially integrate behavioral readouts

Allergic, Pathogenic, and Airway Injury Models
on the Core IACUC Protocol

Do you have experience with models that induce lung damage or airway hyperresponsiveness? If you don’t, we have experience with the following models below and they are included on our IACUC protocol.  Consult with the Core to determine your transfer needs.

  • OVA (ovalbumin)
  • HDM (house dust mite)
  • Papain
  • LPS
  • Fungal Antigen (Alternaria alternata, Aspergillus fumigatus)
  • Helminth Induced Lung Injury (Nippostrongylus brasiliensis, Strongyloides ratti)
  • Bleomycin Induced Lung Injury

Additional Infectious and Metabolic Disease Models Used

We have worked with investigators using additional models of lung injury, inflammation, and airway hyper responsiveness:

  • Sendai Virus Induced Lung Injury
  • Influenza Induced Lung Injury
  • High fat fed diet induced obesity (HFD), alone and with HMD allergy model