New Bolton Center Kennett Square, PA
Emergencies & Appointments:
Ryan Hospital Philadelphia, PA


Diplomate, American College of Veterinary Pathology (Anatomic Pathology)

Assistant Professor of Comparative Pathology, University of Pennsylvania School of Veterinary Medicine

Technical Director of the Comparative Pathology Core, University of Pennsylvania, School of Veterinary Medicine
Contact Information:

My main research interest is the pathological evaluation of laboratory animals used in fundamental research and preclinical studies. A major focus of this research is the assessment of effects and toxicities of immunotherapies and genetic engineering. I am also passionate about developing the field of image analysis on digital slides for data quantification and analysis. Diagnostically, I have a strong interest in ocular pathology and oversee the ocular pathology service within the Penn Vet Diagnostic Laboratory.

Bertram, C. A., Aubreville, M., Donovan, T. A., Bartel, A., Wilm, F., Marzahl, C., Assenmacher, C. A., Becker, K., Bennett, M., Corner, S., Cossic, B., Denk, D., Dettwiler, M., Gonzalez, B. G., Gurtner, C., Haverkamp, A. K., Heier, A., Lehmbecker, A., Merz, S., Noland, E. L., Plog, S., Schmidt, A., Sebastian, F., Sledge, D. G., Smedley, R. C., Tecilla, M., Thaiwong, T., Fuchs-Baumgartinger, A., Meuten, D. J., Breininger, K., Kiupel, M., Maier, A., Klopfleisch, R. Computer-assisted mitotic count using a deep learning-based algorithm improves interobserver reproducibility and accuracy Veterinary Pathology 59: 211-226, 2022.

Lenz, J. A., Assenmacher, C. A., Costa, V., Louka, K., Rau, S., Keuler, N. S., Zhang, P. J., Maki, R. G., Durham, A. C., Radaelli, E., Atherton, M. J. Increased tumor-infiltrating lymphocyte density is associated with favorable outcomes in a comparative study of canine histiocytic sarcoma Cancer Immunology, Immunotherapy 71: 807-818, 2022.

Sultan, L. R., Cary, T. W., Al-Hasani, M., Karmacharya, M. B., Venkatesh, S. S., Assenmacher, C. A., Radaelli, E., Sehgal, C. M. Can Sequential Images from the Same Object Be Used for Training Machine Learning Models? A Case Study for Detecting Liver Disease by Ultrasound Radiomics AI (Basel) 3: 739-750, 2022.

Assenmacher, C. A., Santagostino, S. F., Oyama, M. A., Marine, J. C., Bonvin, E., Radaelli, E. Classification and Grading of Melanocytic Lesions in a Mouse Model of NRAS-driven Melanomagenesis Journal of Histochemistry and Cytochemistry 69: 203-218, 2021.

Breda, L., Ghiaccio, V., Tanaka, N., Jarocha, D., Ikawa, Y., Abdulmalik, O., Dong, A., Casu, C., Raabe, T. D., Shan, X., Danet-Desnoyers, G. A., Doto, A. M., Everett, J., Bushman, F. D., Radaelli, E., Assenmacher, C. A., Tarrant, J. C., Hoepp, N., Kurita, R., Nakamura, Y., Guzikowski, V., Smith-Whitley, K., Kwiatkowski, J. L., Rivella, S. Lentiviral vector ALS20 yields high hemoglobin levels with low genomic integrations for treatment of beta-globinopathies Molecular Therapy 29: 1625-1638, 2021.

Chen, K. W., Demarco, B., Ramos, S., Heilig, R., Goris, M., Grayczyk, J. P., Assenmacher, C. A., Radaelli, E., Joannas, L. D., Henao-Mejia, J., Tacchini-Cottier, F., Brodsky, I. E., Broz, P. RIPK1 activates distinct gasdermins in macrophages and neutrophils upon pathogen blockade of innate immune signaling Proceedings of the National Academy of Sciences of the United States of America 118: , 2021.

Church, M. E., Ceja, G., McGeehan, M., Miller, M. C., Farias, P., Sanchez, M. D., Swain, G. P., Assenmacher, C. A., Stopa, E. G., Vite, C. H., Bar-Or, A., Alvarez, J. I. Meningeal B Cell Clusters Correlate with Submeningeal Pathology in a Natural Model of Multiple Sclerosis Journal of Immunology 207: 44-54, 2021.

Nguyen, G. N., Everett, J. K., Kafle, S., Roche, A. M., Raymond, H. E., Leiby, J., Wood, C., Assenmacher, C. A., Merricks, E. P., Long, C. T., Kazazian, H. H., Nichols, T. C., Bushman, F. D., Sabatino, D. E. A long-term study of AAV gene therapy in dogs with hemophilia A identifies clonal expansions of transduced liver cells Nature Biotechnology 39: 47-55, 2021.

Santagostino, S. F., Assenmacher, C. A., Tarrant, J. C., Adedeji, A. O., Radaelli, E. Mechanisms of Regulated Cell Death: Current Perspectives Veterinary Pathology 58: 596-623, 2021.

Tarrant, J. C., Binder, Z. A., Bugatti, M., Vermi, W., van den Oord, J., Ranieri, B., Assenmacher, C. A., Hoepp, N., O'Rourke, D. M., Shan, X., Danet-Desnoyers, G., Radaelli, E. Pathology of macrophage activation syndrome in humanized NSGS mice Research in Veterinary Science 134: 137-146, 2021.

Velalopoulou, A., Karagounis, I. V., Cramer, G. M., Kim, M. M., Skoufos, G., Goia, D., Hagan, S., Verginadis,, II, Shoniyozov, K., Chiango, J., Cerullo, M., Varner, K., Yao, L., Qin, L., Hatzigeorgiou, A. G., Minn, A. J., Putt, M., Lanza, M., Assenmacher, C. A., Radaelli, E., Huck, J., Diffenderfer, E., Dong, L., Metz, J., Koumenis, C., Cengel, K. A., Maity, A., Busch, T. M. FLASH Proton Radiotherapy Spares Normal Epithelial and Mesenchymal Tissues While Preserving Sarcoma Response Cancer Research 81: 4808-4821, 2021.

Zhang, Q., Hresko, M. E., Picton, L. K., Su, L., Hollander, M. J., Nunez-Cruz, S., Zhang, Z., Assenmacher, C. A., Sockolosky, J. T., Garcia, K. C., Milone, M. C. A human orthogonal IL-2 and IL-2Rbeta system enhances CAR T cell expansion and antitumor activity in a murine model of leukemia Science Translational Medicine 13: eabg6986, 2021.

Aghajanian, H., Kimura, T., Rurik, J. G., Hancock, A. S., Leibowitz, M. S., Li, L., Scholler, J., Monslow, J., Lo, A., Han, W., Wang, T., Bedi, K., Morley, M. P., Linares Saldana, R. A., Bolar, N. A., McDaid, K., Assenmacher, C. A., Smith, C. L., Wirth, D., June, C. H., Margulies, K. B., Jain, R., Pure, E., Albelda, S. M., Epstein, J. A. Targeting cardiac fibrosis with engineered T cells Nature 573: 430-433, 2019.

DVM University of Liège, School of Veterinary Medicine, Liège, Belgium, 2012

Diplomate, American College of Veterinary Pathology (Anatomic Pathology)

University of Liege, School of Veterinary Medicine, Liege, Belgium (2012 to 2013)
Combined Small animal rotating internship and Master of Science (MSc)

University of Pennsylvania, School of Veterinary Medicine Philadelphia, PA (2015 to 2018)
Residency in Veterinary Anatomic Pathology

University of Pennsylvania, School of Veterinary Medicine Philadelphia, PA (2018 to 2020)
Fellowship/Lecturer at the Comparative Pathology Core

The Animal Health Trust, Newmarket, UK (2013 to 2014)
Small animal rotating internship