CHRISTOPHER J. LENGNER
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New Bolton Center Kennett Square, PA
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610-444-5800
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Ryan Hospital Philadelphia, PA
Emergencies:
215-746-8911
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215-746-8387
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CHRISTOPHER J. LENGNER, B.S., Ph.D.

Associate Professor, Department of Biomedical Sciences

Member, Institute for Regenerative Medicine, Institute for Regenerative Medicine

Member, Center for Molecular Studies in Digestive and Liver Diseases, NIH P30 Center for Molecular Studies in Digestive and Liver Diseases, School of Medicine

Associate Director, Institute for Regenerative Medicine

Director, Center for Animal Transgenesis

Research Areas: Regenerative medicine, Cancer, Reprogramming, Oncogenesis, Oncogenic transformation, Somatic stem cells, Oncology, epithelium, regeneration
PubMed Link
Contact Information:
Christopher J. Lengner
Associate Professor
Department of Biomedical Sciences
School of Veterinary Medicine
3800 Spruce Street, Room 390EA

The overarching goal of my research program is to gain an understanding of the molecular mechanisms that govern stem cell potency and how dysregulation of these mechanisms can contribute to disease onset and progression, including oncogenesis. My laboratory employs genetic, genomic, and single-cell analysis approaches in murine systems to address these fundamental questions, and, in parallel, utilizes human induced pluripotent cells and patient samples to translate findings into human systems. Using these approaches we have identified novel pathways underlying the somatic stem cell self-renewal and the ontogeny of hematopoietic and gastrointestinal cancers. These pathways are subsequently being assessed as potential points for therapeutic intervention in cancers and diseases of regenerative failure. We have also begun to unravel the hierarchical structure of the intestinal stem cell compartment with the ultimate goal of understanding how perturbations in this hierarchy contribute to disease states, including cancer, chronic inflammation, and regeneration in response to acute injuries such as ischemia or radiation damage.

Lab Members

Ning Li, M.D., Postdoctoral Fellow
Dong-Hun Woo, Ph.D., Postdoctoral Fellow
Angela Ddamba, Research Specialist
Ryan Cedeno, Graduate Student
Maryam Yousefi, Graduate Student (HHMI International Fellow)
Carla Hoge, Undergraduate (UPenn Vagelos Scholar)


Park Hanseul, Oh Jungju, Shim Gayong, Cho Byounggook, Chang Yujung, Kim Siyoung, Baek Soonbong, Kim Hongwon, Shin Jeain, Choi Hwan, Yoo Junsang, Kim Junyeop, Jun Won, Lee Minhyung, Lengner Christopher J, Oh Yu-Kyoung, Kim Jongpil In vivo neuronal gene editing via CRISPR-Cas9 amphiphilic nanocomplexes alleviates deficits in mouse models of Alzheimer's disease. Nature neuroscience 22: 524-528, 2019.

Kim Hongwon, Park Hyeok Ju, Choi Hwan, Chang Yujung, Park Hanseul, Shin Jaein, Kim Junyeop, Lengner Christopher J, Lee Yong Kyu, Kim Jongpil Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids. Stem cell reports 12: 518-531, 2019.

Tian Yuhua, Xu Jiuzhi, Li Yuan, Zhao Ran, Du Sujuan, Lv Cong, Wu Wei, Liu Ruiqi, Sheng Xiaole, Song Yongli, Bi Xueyun, Li Guilin, Li Mengzhen, Wu Xi, Lou Pengbo, You Huiwen, Cui Wei, Sun Jinyue, Shuai Jianwei, Ren Fazheng, Zhang Bing, Guo Mingzhou, Hou Xiaohua, Wu Kaichun, Xue Lixiang, Zhang Hongquan, Plikus Maksim V, Cong Yingzi, Lengner Christopher J, Liu Zhanju, Yu Zhengquan MicroRNA-31 Reduces Inflammatory Signaling and Promotes Regeneration in Colon Epithelium, and Delivery of Mimics in Microspheres Reduces Colitis in Mice. Gastroenterology : , 2019.

Palzer Laura, Bader Jessica J, Angel Frances, Witzel Megan, Blaser Sydney, McNeil Alexis, Wandersee Miles K, Leu N Adrian, Lengner Christopher J, Cho Clara E, Welch Kevin D, Kirkland James B, Meyer Ralph G, Meyer-Ficca Mirella L Alpha-Amino-Beta-Carboxy-Muconate-Semialdehyde Decarboxylase Controls Dietary Niacin Requirements for NAD Synthesis. Cell reports 25: 1359-1370.e4, 2018.

Godfrey Tanner C, Wildman Benjamin J, Beloti Marcio M, Kemper Austin G, Ferraz Emanuela P, Roy Bhaskar, Rehan Mohammad, Afreen Lubana H, Kim Eddy, Lengner Christopher J, Hassan Quamarul The microRNA-23a cluster regulates the developmental HoxA cluster function during osteoblast differentiation. The Journal of biological chemistry 293: 17646-17660, 2018.

Chatterji Priya, Hamilton Kathryn E, Liang Shun, Andres Sarah F, Wijeratne H R Sagara, Mizuno Rei, Simon Lauren A, Hicks Philip D, Foley Shawn W, Pitarresi Jason R, Klein-Szanto Andres J, Mah Amanda T, Van Landeghem Laurianne, Gregory Brian D, Lengner Christopher J, Madison Blair B, Shah Premal, Rustgi Anil K The LIN28B-IMP1 post-transcriptional regulon has opposing effects on oncogenic signaling in the intestine. Genes & development 32: 1020-1034, 2018.

Giroux Véronique, Stephan Julien, Chatterji Priya, Rhoades Ben, Wileyto E Paul, Klein-Szanto Andres J, Lengner Christopher J, Hamilton Kathryn E, Rustgi Anil K Mouse Intestinal Krt15+ Crypt Cells Are Radio-Resistant and Tumor Initiating. Stem cell reports 10: 1947-1958, 2018.

Godfrey Tanner C, Wildman Benjamin J, Javed Amjad, Lengner Christopher J, Hassan Mohammad Quamarul Epigenetic remodeling and modification to preserve skeletogenesis in vivo. Connective tissue research 59: 52-54, 2018.

Yousefi Maryam, Nakauka-Ddamba Angela, Berry Corbett T, Li Ning, Schoenberger Jenna, Simeonov Kamen P, Cedeno Ryan J, Yu Zhengquan, Lengner Christopher J Calorie Restriction Governs Intestinal Epithelial Regeneration through Cell-Autonomous Regulation of mTORC1 in Reserve Stem Cells. Stem cell reports 10: 703-711, 2018.

Yoo, J., Lee, E., Kim, H. Y., Youn, D. H., Jung, J., Kim, H., Chang, Y., Lee, W., Shin, J., Baek, S., Jang, W., Jun, W., Kim, S., Hong, J., Park, H. J., Lengner, C. J., Moh, S. H., Kwon, Y., Kim, J. Electromagnetized gold nanoparticles mediate direct lineage reprogramming into induced dopamine neurons in vivo for Parkinson''s disease therapy Nat Nanotechnol 12: 1006-1014, 2017.

Yousefi, M., Li, L., Lengner, C. J. Hierarchy and Plasticity in the Intestinal Stem Cell Compartment Trends Cell Biol 27: 753-764, 2017.

Yang, T. B., Chen, Q., Deng, J. T., Jagannathan, G., Tobias, J. W., Schultz, D. C., Wang, S., Lengner, C. J., Rustgi, A. K., Lynch, J. P., Johnson, F. B. Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche Nat Commun 8: 14766, 2017.

Lv, C., Li, F., Li, X., Tian, Y., Zhang, Y., Sheng, X., Song, Y., Meng, Q., Yuan, S., Luan, L., Andl, T., Feng, X., Jiao, B., Xu, M., Plikus, M. V., Dai, X., Lengner, C., Cui, W., Ren, F., Shuai, J., Millar, S. E., Yu, Z. MiR-31 promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonists Nat Commun 8: 1036, 2017.

Ma, X., Tian, Y., Song, Y., Shi, J., Xu, J., Xiong, K., Li, J., Xu, W., Zhao, Y., Shuai, J., Chen, L., Plikus, M. V., Lengner, C. J., Ren, F., Xue, L., Yu, Z. Msi2 Maintains Quiescent State of Hair Follicle Stem Cells by Directly Repressing the Hh Signaling Pathway J Invest Dermatol 137: 1015-1024, 2017.

Nakauka-Ddamba, A., Lengner, C. J. Gut with the Program: Direct Reprogramming toward Intestinal Epithelium Realized Cell Stem Cell 21: 417-418, 2017.

Tian, Y., Ma, X., Lv, C., Sheng, X., Li, X., Zhao, R., Song, Y., Andl, T., Plikus, M. V., Sun, J., Ren, F., Shuai, J., Lengner, C. J., Cui, W., Yu, Z. Stress responsive miR-31 is a major modulator of mouse intestinal stem cells during regeneration and tumorigenesis Elife 6: , 2017.

Vu, L. P., Prieto, C., Amin, E. M., Chhangawala, S., Krivtsov, A., Calvo-Vidal, M. N., Chou, T., Chow, A., Minuesa, G., Park, S. M., Barlowe, T. S., Taggart, J., Tivnan, P., Deering, R. P., Chu, L. P., Kwon, J. A., Meydan, C., Perales-Paton, J., Arshi, A., Gonen, M., Famulare, C., Patel, M., Paietta, E., Tallman, M. S., Lu, Y., Glass, J., Garret-Bakelman, F. E., Melnick, A., Levine, R., Al-Shahrour, F., Jaras, M., Hacohen, N., Hwang, A., Garippa, R., Lengner, C. J., Armstrong, S. A., Cerchietti, L., Cowley, G. S., Root, D., Doench, J., Leslie, C., Ebert, B. L., Kharas, M. G. Functional screen of MSI2 interactors identifies an essential role for SYNCRIP in myeloid leukemia stem cells Nat Genet 49: 866-875, 2017.

Cedeno, R. J., Nakauka-Ddamba, A., Yousefi, M., Sterling, S., Leu, N. A., Li, N., Pehrson, J. R., Lengner, C. J. The histone variant macroH2A confers functional robustness to the intestinal stem cell compartment PLoS One 12: e0185196, 2017.

Giroux, V., Lento, A. A., Islam, M., Pitarresi, J. R., Kharbanda, A., Hamilton, K. E., Whelan, K. A., Long, A., Rhoades, B., Tang, Q., Nakagawa, H., Lengner, C. J., Bass, A. J., Wileyto, E. P., Klein-Szanto, A. J., Wang, T. C., Rustgi, A. K. Long-lived keratin 15+ esophageal progenitor cells contribute to homeostasis and regeneration J Clin Invest 127: 2378-2391, 2017.

Kharas, M. G., Lengner, C. J. Stem Cells, Cancer, and MUSASHI in Blood and Guts Trends Cancer 3: 347-356, 2017.

Kim, H., Yoo, J., Shin, J., Chang, Y., Jung, J., Jo, D. G., Kim, J., Jang, W., Lengner, C. J., Kim, B. S., Kim, J. Modelling APOE varepsilon3/4 allele-associated sporadic Alzheimer''s disease in an induced neuron Brain 140: 2193-2209, 2017.

Katlinskaya, Y. V., Katlinski, K. V., Lasri, A., Li, N., Beiting, D. P., Durham, A. C., Yang, T., Pikarsky, E., Lengner, C. J., Johnson, F. B., Ben-Neriah, Y., Fuchs, S. Y. Type I Interferons Control Proliferation and Function of the Intestinal Epithelium Mol Cell Biol 36: 1124-35, 2016.

Cieply, B., Park, J. W., Nakauka-Ddamba, A., Bebee, T. W., Guo, Y., Shang, X., Lengner, C. J., Xing, Y., Carstens, R. P. Multiphasic and Dynamic Changes in Alternative Splicing during Induction of Pluripotency Are Coordinated by Numerous RNA-Binding Proteins Cell Rep 15: 247-55, 2016.

Taggart, J., Ho, T. C., Amin, E., Xu, H., Barlowe, T. S., Perez, A. R., Durham, B. H., Tivnan, P., Okabe, R., Chow, A., Vu, L., Park, S. M., Prieto, C., Famulare, C., Patel, M., Lengner, C. J., Verma, A., Roboz, G., Guzman, M., Klimek, V. M., Abdel-Wahab, O., Leslie, C., Nimer, S. D., Kharas, M. G. MSI2 is required for maintaining activated myelodysplastic syndrome stem cells Nat Commun 7: 10739, 2016.

Li, N., Yousefi, M., Nakauka-Ddamba, A., Tobias, J. W., Jensen, S. T., Morrisey, E. E., Lengner, C. J. Heterogeneity in readouts of canonical wnt pathway activity within intestinal crypts Dev Dyn 245: 822-33, 2016.

Li, N., Nakauka-Ddamba, A., Tobias, J., Jensen, S. T., Lengner, C. J. Mouse Label-Retaining Cells Are Molecularly and Functionally Distinct From Reserve Intestinal Stem Cells Gastroenterology 151: 298-310 e7, 2016.

Woo, D. H., Chen, Q., Yang, T. L., Glineburg, M. R., Hoge, C., Leu, N. A., Johnson, F. B., Lengner, C. J. Enhancing a Wnt-Telomere Feedback Loop Restores Intestinal Stem Cell Function in a Human Organotypic Model of Dyskeratosis Congenita Cell Stem Cell 19: 397-405, 2016.

Zhang, Y., Li, F., Song, Y., Sheng, X., Ren, F., Xiong, K., Chen, L., Zhang, H., Liu, D., Lengner, C. J., Xue, L., Yu, Z. Numb and Numbl act to determine mammary myoepithelial cell fate, maintain epithelial identity, and support lactogenesis FASEB J 30: 3474-3488, 2016.

Yousefi, M., Li, N., Nakauka-Ddamba, A., Wang, S., Davidow, K., Schoenberger, J., Yu, Z., Jensen, S. T., Kharas, M. G., Lengner, C. J. Msi RNA-binding proteins control reserve intestinal stem cell quiescence J Cell Biol 215: 401-413, 2016.

Yu, Q., Katlinskaya, Y. V., Carbone, C. J., Zhao, B., Katlinski, K. V., Zheng, H., Guha, M., Li, N., Chen, Q., Yang, T., Lengner, C. J., Greenberg, R. A., Johnson, F. B., Fuchs, S. Y. DNA-damage-induced type I interferon promotes senescence and inhibits stem cell function Cell Rep 11: 785-797, 2015.

Yuan, S., Li, F., Meng, Q., Zhao, Y., Chen, L., Zhang, H., Xue, L., Zhang, X., Lengner, C., Yu, Z. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22 PLoS Genet 11: e1005253, 2015.

Li, N., Yousefi, M., Nakauka-Ddamba, A., Li, F., Vandivier, L., Parada, K., Woo, D. H., Wang, S., Naqvi, A. S., Rao, S., Tobias, J., Cedeno, R. J., Minuesa, G., Y, K., Barlowe, T. S., Valvezan, A., Shankar, S., Deering, R. P., Klein, P. S., Jensen, S. T., Kharas, M. G., Gregory, B. D., Yu, Z., Lengner, C. J. The Msi Family of RNA-Binding Proteins Function Redundantly as Intestinal Oncoproteins Cell Rep 13: 2440-2455, 2015.

Park, S. M., Gonen, M., Vu, L., Minuesa, G., Tivnan, P., Barlowe, T. S., Taggart, J., Lu, Y., Deering, R. P., Hacohen, N., Figueroa, M. E., Paietta, E., Fernandez, H. F., Tallman, M. S., Melnick, A., Levine, R., Leslie, C., Lengner, C. J., Kharas, M. G. Musashi2 sustains the mixed-lineage leukemia-driven stem cell regulatory program J Clin Invest 125: 1286-98, 2015.

Park, H., Kim, H., Yoo, J., Lee, J., Choi, H., Baek, S., Lee, C. J., Kim, J., Lengner, C. J., Sung, J. S., Kim, J. Homogeneous generation of iDA neurons with high similarity to bona fide DA neurons using a drug inducible system Biomaterials 72: 152-62, 2015.

Wang, S., Li, N., Yousefi, M., Nakauka-Ddamba, A., Li, F., Parada, K., Rao, S., Minuesa, G., Katz, Y., Gregory, B. D., Kharas, M. G., Yu, Z., Lengner, C. J. Transformation of the intestinal epithelium by the MSI2 RNA-binding protein Nat Commun 6: 6517, 2015.

Wang, Shan; Li, Ning; Yousefi, Maryam; Nakauka-Ddamba, Angela; Li, Fan; Parada, Kimberly; Rao, Shilpa; Minuesa, Gerard; Katz, Yarden; Gregory, Brian D; Kharas, Michael G; Yu, Zhengquan; Lengner, Christopher J. Transformation of the intestinal epithelium by the MSI2 RNA-binding protein. Nature communications 6: 6517, 2015.

Park, Sun-Mi; Gonen, Mithat; Vu, Ly; Minuesa, Gerard; Tivnan, Patrick; Barlowe, Trevor S; Taggart, James; Lu, Yuheng; Deering, Raquel P; Hacohen, Nir; Figueroa, Maria E; Paietta, Elisabeth; Fernandez, Hugo F; Tallman, Martin S; Melnick, Ari; Levine, Ross; Leslie, Christina; Lengner, Christopher J; Kharas, Michael G. Musashi2 sustains the mixed-lineage leukemia-driven stem cell regulatory program. Journal of Clinical Investigation 125: 1286-98, 2015.

Park, Sun-Mi; Deering, Raquel P; Lu, Yuheng; Tivnan, Patrick; Lianoglou, Steve; Al-Shahrour, Fatima; Ebert, Benjamin L; Hacohen, Nir; Leslie, Christina; Daley, George Q; Lengner, Christopher J; Kharas, Michael G. Musashi-2 controls cell fate, lineage bias, and TGF-beta signaling in HSCs. Journal of Experimental Medicine 211: 71-87, 2014.

Valvezan, Alexander J; Huang, Jian; Lengner, Christopher J; Pack, Michael; Klein, Peter S. Oncogenic mutations in adenomatous polyposis coli (Apc) activate mechanistic target of rapamycin complex 1 (mTORC1) in mice and zebrafish. Disease Models & Mechanisms 7: 63-71, 2014.

Li, Ning; Yousefi, Maryam; Nakauka-Ddamba, Angela; Jain, Rajan; Tobias, John; Epstein, Jonathan A; Jensen, Shane T; Lengner, Christopher J. Single-cell analysis of proxy reporter allele-marked epithelial cells establishes intestinal stem cell hierarchy. Stem Cell Reports 3: 876-91, 2014.

Baek, Soonbong; Quan, Xiaoyuan; Kim, Soochan; Lengner, Christopher; Park, Jung-Keug; Kim, Jongpil. Electromagnetic fields mediate efficient cell reprogramming into a pluripotent state. Acs Nano 8: 10125-38, 2014.

Katz, Yarden; Li, Feifei; Lambert, Nicole J; Sokol, Ethan S; Tam, Wai-Leong; Cheng, Albert W; Airoldi, Edoardo M; Lengner, Christopher J; Gupta, Piyush B; Yu, Zhengquan; Jaenisch, Rudolf; Burge, Christopher B. Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state. eLife 3: e03915, 2014.

Katz, Y., Li, F., Lambert, N. J., Sokol, E. S., Tam, W. L., Cheng, A. W., Airoldi, E. M., Lengner, C. J., Gupta, P. B., Yu, Z., Jaenisch, R., Burge, C. B. Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state Elife 3: e03915, 2014.

Valvezan, A. J., Huang, J., Lengner, C. J., Pack, M., Klein, P. S. Oncogenic mutations in adenomatous polyposis coli (Apc) activate mechanistic target of rapamycin complex 1 (mTORC1) in mice and zebrafish Dis Model Mech 7: 63-71, 2014.

Park, S. M., Deering, R. P., Lu, Y., Tivnan, P., Lianoglou, S., Al-Shahrour, F., Ebert, B. L., Hacohen, N., Leslie, C., Daley, G. Q., Lengner, C. J., Kharas, M. G. Musashi-2 controls cell fate, lineage bias, and TGF-beta signaling in HSCs J Exp Med 211: 71-87, 2014.

Baek, S., Quan, X., Kim, S., Lengner, C., Park, J. K., Kim, J. Electromagnetic fields mediate efficient cell reprogramming into a pluripotent state ACS Nano 8: 10125-38, 2014.

Li, N., Yousefi, M., Nakauka-Ddamba, A., Jain, R., Tobias, J., Epstein, J. A., Jensen, S. T., Lengner, C. J. Single-cell analysis of proxy reporter allele-marked epithelial cells establishes intestinal stem cell hierarchy Stem Cell Reports 3: 876-91, 2014.

Yildirim, O., Hung, J. H., Cedeno, R. J., Weng, Z., Lengner, C. J., Rando, O. J. A system for genome-wide histone variant dynamics in ES cells reveals dynamic MacroH2A2 replacement at promoters PLoS Genet 10: e1004515, 2014.

Feng, R., Lengner, C. Application of Stem Cell Technology in Dental Regenerative Medicine Adv Wound Care (New Rochelle) 2: 296-305, 2013.

Ghule, P. N., Medina, R., Lengner, C. J., Mandeville, M., Qiao, M., Dominski, Z., Lian, J. B., Stein, J. L., van Wijnen, A. J., Stein, G. S. Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells J Cell Physiol 226: 1149-56, 2011.

Kim, J., Lengner, C. J., Kirak, O., Hanna, J., Cassady, J. P., Lodato, M. A., Wu, S., Faddah, D. A., Steine, E. J., Gao, Q., Fu, D., Dawlaty, M., Jaenisch, R. Reprogramming of postnatal neurons into induced pluripotent stem cells by defined factors Stem Cells 29: 992-1000, 2011.

Kim, J., Su, S. C., Wang, H., Cheng, A. W., Cassady, J. P., Lodato, M. A., Lengner, C. J., Chung, C. Y., Dawlaty, M. M., Tsai, L. H., Jaenisch, R. Functional integration of dopaminergic neurons directly converted from mouse fibroblasts Cell Stem Cell 9: 413-9, 2011.

Kim, Jongpil; Su, Susan C; Wang, Haoyi; Cheng, Albert W; Cassady, John P; Lodato, Michael A; Lengner, Christopher J; Chung, Chee-Yeun; Dawlaty, Meelad M; Tsai, Li-Huei; Jaenisch, Rudolf. Functional integration of dopaminergic neurons directly converted from mouse fibroblasts. Cell Stem Cell 9: 413-9, 2011.

Liu, Julie C; Lengner, Christopher J; Gaur, Tripti; Lou, Yang; Hussain, Sadiq; Jones, Marci D; Borodic, Brent; Colby, Jennifer L; Steinman, Heather A; van Wijnen, Andre J; Stein, Janet L; Jones, Stephen N; Stein, Gary S; Lian, Jane B. Runx2 protein expression utilizes the Runx2 P1 promoter to establish osteoprogenitor cell number for normal bone formation. Journal of Biological Chemistry 286: 30057-70, 2011.

Kim, Jongpil; Lengner, Christopher J; Kirak, Oktay; Hanna, Jacob; Cassady, John P; Lodato, Michael A; Wu, Su; Faddah, Dina A; Steine, Eveline J; Gao, Qing; Fu, Dongdong; Dawlaty, Meelad; Jaenisch, Rudolf. Reprogramming of postnatal neurons into induced pluripotent stem cells by defined factors. Stem Cells 29: 992-1000, 2011.

Liu, J. C., Lengner, C. J., Gaur, T., Lou, Y., Hussain, S., Jones, M. D., Borodic, B., Colby, J. L., Steinman, H. A., van Wijnen, A. J., Stein, J. L., Jones, S. N., Stein, G. S., Lian, J. B. Runx2 protein expression utilizes the Runx2 P1 promoter to establish osteoprogenitor cell number for normal bone formation J Biol Chem 286: 30057-70, 2011.

Ghule Prachi N, Medina Ricardo, Lengner Chris J, Mandeville Matthew, Qiao Meng, Dominski Zbigniew, Lian Jane B, Stein Janet L, van Wijnen Andre J, Stein Gary S Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells. Journal of cellular physiology : , 2010.

Kharas Michael G*, Lengner Christopher J*, Al-Shahrour Fatima, Bullinger Lars, Ball Brian, Zaidi Samir, Morgan Kelly, Tam Winnie, Paktinat Mahnaz, Okabe Rachel, Gozo Maricel, Einhorn William, Lane Steven W, Scholl Claudia, Fröhling Stefan, Fleming Mark, Ebert Benjamin L, Gilliland D Gary, Jaenisch Rudolf, Daley George Q *equally contributing authors Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia. Nature medicine 16: 903-8, 2010.

Lengner Christopher J, Gimelbrant Alexander A, Erwin Jennifer A, Cheng Albert Wu, Guenther Matthew G, Welstead G Grant, Alagappan Raaji, Frampton Garrett M, Xu Ping, Muffat Julien, Santagata Sandro, Powers Doug, Barrett C Brent, Young Richard A, Lee Jeannie T, Jaenisch Rudolf, Mitalipova Maisam Derivation of pre-X inactivation human embryonic stem cells under physiological oxygen concentrations. Cell 141: 872-83, 2010.

Hanna Jacob, Cheng Albert W, Saha Krishanu, Kim Jongpil, Lengner Christopher J, Soldner Frank, Cassady John P, Muffat Julien, Carey Bryce W, Jaenisch Rudolf Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Proceedings of the National Academy of Sciences of the United States of America 107: 9222-7, 2010.

Lengner Christopher J iPS cell technology in regenerative medicine. Annals of the New York Academy of Sciences 1192: 38-44, 2010.

Lengner, C. J. iPS cell technology in regenerative medicine Ann N Y Acad Sci 1192: 38-44, 2010.

Lengner, C. J., Gimelbrant, A. A., Erwin, J. A., Cheng, A. W., Guenther, M. G., Welstead, G. G., Alagappan, R., Frampton, G. M., Xu, P., Muffat, J., Santagata, S., Powers, D., Barrett, C. B., Young, R. A., Lee, J. T., Jaenisch, R., Mitalipova, M. Derivation of pre-X inactivation human embryonic stem cells under physiological oxygen concentrations Cell 141: 872-83, 2010.

Kharas, M. G., Lengner, C. J., Al-Shahrour, F., Bullinger, L., Ball, B., Zaidi, S., Morgan, K., Tam, W., Paktinat, M., Okabe, R., Gozo, M., Einhorn, W., Lane, S. W., Scholl, C., Frohling, S., Fleming, M., Ebert, B. L., Gilliland, D. G., Jaenisch, R., Daley, G. Q. Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia Nat Med 16: 903-8, 2010.

Hanna, J., Cheng, A. W., Saha, K., Kim, J., Lengner, C. J., Soldner, F., Cassady, J. P., Muffat, J., Carey, B. W., Jaenisch, R. Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs Proc Natl Acad Sci U S A 107: 9222-7, 2010.

Hanna, J., Markoulaki, S., Mitalipova, M., Cheng, A. W., Cassady, J. P., Staerk, J., Carey, B. W., Lengner, C. J., Foreman, R., Love, J., Gao, Q., Kim, J., Jaenisch, R. Metastable pluripotent states in NOD-mouse-derived ESCs Cell Stem Cell 4: 513-24, 2009.

Hanna, J., Saha, K., Pando, B., van Zon, J., Lengner, C. J., Creyghton, M. P., van Oudenaarden, A., Jaenisch, R. Direct cell reprogramming is a stochastic process amenable to acceleration Nature 462: 595-601, 2009.

Hanna Jacob, Saha Krishanu, Pando Bernardo, van Zon Jeroen, Lengner Christopher J, Creyghton Menno P, van Oudenaarden Alexander, Jaenisch Rudolf Direct cell reprogramming is a stochastic process amenable to acceleration. Nature 462: 595-601, 2009.

Hanna Jacob, Markoulaki Styliani, Mitalipova Maisam, Cheng Albert W, Cassady John P, Staerk Judith, Carey Bryce W, Lengner Christopher J, Foreman Ruth, Love Jennifer, Gao Qing, Kim Jongpil, Jaenisch Rudolf Metastable pluripotent states in NOD-mouse-derived ESCs. Cell stem cell 4: 513-24, 2009.

Markoulaki Styliani, Hanna Jacob, Beard Caroline, Carey Bryce W, Cheng Albert W, Lengner Christopher J, Dausman Jessica A, Fu Dongdong, Gao Qing, Wu Su, Cassady John P, Jaenisch Rudolf Transgenic mice with defined combinations of drug-inducible reprogramming factors. Nature biotechnology 27: 169-71, 2009.

Markoulaki, S., Hanna, J., Beard, C., Carey, B. W., Cheng, A. W., Lengner, C. J., Dausman, J. A., Fu, D., Gao, Q., Wu, S., Cassady, J. P., Jaenisch, R. Transgenic mice with defined combinations of drug-inducible reprogramming factors Nat Biotechnol 27: 169-71, 2009.

B.S. (Biotechnology) Worcester Polytechnic Institute, 1998

Ph.D. (Cell and Molecular Biology) University of Massachusetts Medical School, 2004

Whitehead Institute for Biomedical Research (MIT) (2005 to 2010)
Ruth L. Kirschstein Postdoctoral Fellow, Stem Cell Biology and Genetics