My clinical, diagnostic and research expertise is veterinary anatomic pathology with special focus on orthopedic and gastrointestinal pathology. Current and past research projects have included clinical research, translational animal models of human and animal disease, and exploratory investigations including academic and industrial collaborations.
One focus of my orthopedic research includes translational animal models evaluating bone, joint and tendonous tissues. Current and past projects include analyses of orthopedic surgical procedures, biomaterials, and devices utilizing large animal models of human orthopedic disease including swine, sheep, goats, horses and dogs. Internal collaborations include Penn Vet's Comparative Orthopedic Research Laboratory (CORL: Drs. Dean Richardson, Tom Schaer, and Kyla Ortved) and the Penn Center for Musculoskeletal Disorders (PCMD). A collaboration with Dr. Nicky Mason (Department of Clinical Studies- Philadelphia) investigates a canine model of osteosarcoma investigating T-cell mediated immunotherapy targeting tumor ostoblasts expressing Her2/neu antigen. External collaborations include academic institutions and industry investigating murine fracture healing and gene-therapy for large animal models of naturally occurring osteoarthritis.
Another focus of orthopedic research is equine laminitis, including internal collaborations (Drs. van Eps and Galantino-Homer) and external collaborations (Oklahoma State University, University of Queensland, and Ohio State University). Through correlation of clinical and pathological data with transcriptomic and proteomic data, the goals of the research are 1) to better understand the pathogenesis of laminitis and 2) to identify potential early biomarkers and molecular targets of laminitis for disease prevention and early therapeutic intervention. One unique aspect of the research involves the interaction among different tissue interfaces including epidermal and dermal lamellae, neurovascular structurs, and the distal phalanx.
My secondary research focus involves equine gastrointestinal pathology and the equine intestinal microbiome. Collaborators include Dr. Southwood (Large Animal Emergency and Critical Care service), and Dr. Pitta (Agricultural Systems and Microbial Genomics laboratory) from the Department of Clinical Studies-New Bolton Center. Current and past investigations include establishment of normal parameters for gastrointestinal architecture and resident inflammatory cell populations, evaluation of the effect of peri-operative antimicrobials on the equine fecal microbiome for elective surgical procedures, and the differences in fecal microbiome and intestinal micro-architecture in horses presenting for acute and recurrent colic.
Engiles JB, Stewart HS, Janes J, Kennedy L. A diagnostic pathologist’s guide to carpal disease in racehorses. Journal of Veterinary Diagnostic Investigation 29: 413-430, 2017.Janes JG, Kennedy L, Garrett K, Engiles JB Common pathology of the distal end of the third metacarpal/metatarsal bone in racehorse catastrophic breakdown injuries. Journal of Veterinary Diagnostic Investigation 29: 431-436, 2017.Engiles J B, Galantino-Homer H L, Boston R, McDonald D, Dishowitz M, Hankenson K D Osteopathology in the Equine Distal Phalanx Associated With the Development and Progression of Laminitis. Veterinary pathology : , 2015.Gullbrand Sarah E, Ashinsky Beth G, Bonnevie Edward D, Kim Dong Hwa, Engiles Julie B, Smith Lachlan J, Elliott Dawn M, Schaer Thomas P, Smith Harvey E, Mauck Robert L Long-term mechanical function and integration of an implanted tissue-engineered intervertebral disc. Science translational medicine 10: , 2018.Mason, Nicola, Josephine Gnanandarajah, Julie Engiles, Falon Gray, Danielle Laughlin, Anita Gaurnier-Hausser, Anu Wallecha, Margie Huebner, and Yvonne Paterson. Immunotherapy with a HER2 targeted Listeria induces HER2-specific immunity and demonstrates potential therapeutic effects in a phase I trial in canine osteosarcoma. Clinical Cancer Research : , 2016.Stroud C, Dmitriev I, ... Mason N, Gnanandarajah JS, Engiles JB,...& Harman RJ. A One Health overview, facilitating advances in comparative medicine and translational research. Clinical and Translational Medicine. 5: 26, 2016.Stewart HL, Pitta D, Indugu N, Vecchiarelli B, Engiles JB, Southwood LS. Differences in the equine fecal microbiome using different sampling times and sites. American Journal of Veterinary Research : Accepted Nov, 2017, 2017.Stewart H L, Southwood L L, Indugu N, Vecchiarelli B, Engiles J B, Pitta D Differences in the equine faecal microbiota between horses presenting to a tertiary referral hospital for colic compared to an elective surgical procedure. Equine veterinary journal : , 2018.Stewart Holly L, Engiles Julie B, Stefanovski Darko, Southwood Louise Clinical and intestinal histologic features of horses treated for recurrent colic: 66 cases (2006-2015). Journal of the American Veterinary Medical Association 252: 1279-1288, 2018.Johnstone L, Engiles JB, Tewari D, Johnson A Retrospective Evaluation of Horses Diagnosed with Neuroborreliosis on Postmortem Examination: 16 Cases (2004-2015). Journal of Veterinary Internal Medicine epub doi: 10.1111/jvim.14369: , 2016.
DACVP (Veterinary Anatomic Pathology ) University of Pennsylvania, 2006VMD School of Veterinary Medicine, University of Pennsylvania, 2002B.A. (Biophysics, Dean's Scholar Program) University of Delaware, 1994