MONTSERRAT C. ANGUERA
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New Bolton Center Kennett Square, PA
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Ryan Hospital Philadelphia, PA
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MONTSERRAT C. ANGUERA, Ph.D, B.A

Assistant Professor, Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine

Member, Epigenetics Institute, University of Pennsylvania

Member, Institute for Immunology, University of Pennsylvania

Member, Center for Research on Reproduction & Women's Health, University of Pennsylvania Perelman School of Medicine

Member, Institute for Regenerative Medicine, University of Pennsylvania

Member, CAMB graduate group (DSRB), University of Pennsylvania, Biomedical Graduate Studies

Research Areas: Epigenetics, X-Chromosome Inactivation, Xist RNA, female-biased autoimmunity, epigenetic analyses using female lymphocytes, allele-specific analyses, RNA/DNA FISH, microscopy, long noncoding RNAs
PubMed Link
Contact Information:
Department of Biomedical Sciences
School of Veterinary Medicine
3800 Spruce St., Room 390EB

RESEARCH INTERESTS:

The research in the Anguera laboratory focuses on maintenance of X-chromosome Inactivation in the immune system and in stem cells. They also study epigenetic mechanisms involving long noncoding RNAs during early human development and placental progenitors.

RESEARCH AREAS:

Mechanisms of X-chromosome Inactivation:
We are investigating the molecular mechanisms of X-Chromosome Inactivation, and how altered dosage of X-linked genes affects early embryonic development and contributes to sex-biased disease. We focus on the autoimmune disorder lupus, which has a strong female-bias, exhibits overexpression of X-linked immune-related genes, and involves lymphocytes. We study the epigenetic status of the inactive X in female lymphocytes from humans and mice, and have made the remarkable discovery that these cells do not maintain X-Chromosome Inactivation in the same way as other female somatic cells. We were the first to discover that the inactive X has euchromatic features in female lymphocytes, which may explain the female bias in autoimmune disorders such as lupus. We also study the dynamic mechanisms of Xist RNA localization and heterochromatin mark recruitment to the inactive X following lymphocyte activation.

Long noncoding RNAs during early human development:
We are also investigating sex-specific differences during human placental development using in vitro model systems. We discovered a novel X-linked long noncoding RNA specifically expressed in human placental progenitor cells that regulates the innate immune response.

KEY WORDS

X-chromosome Inactivation, Xist RNA, epigenetics, heterochromatin, female-biased autoimmunity, B cell development, B cell quiescence and activation, T cell quiescence and activation.


WEBSITE
http://www.vet.upenn.edu/research/research-laboratories/research-laboratory/anguera-laboratory

Hantsoo Liisa, Kornfield Sara, Anguera Montserrat C, Epperson C Neill Inflammation: A Proposed Intermediary Between Maternal Stress and Offspring Neuropsychiatric Risk. Biological psychiatry 85: 97-106, 2019.

Syrett Camille M, Sierra Isabel, Berry Corbett L, Beiting Daniel, Anguera Montserrat C Sex-Specific Gene Expression Differences Are Evident in Human Embryonic Stem Cells and During In Vitro Differentiation of Human Placental Progenitor Cells. Stem cells and development 27: 1360-1375, 2018.

Le Coz Carole, Trofa Melissa, Syrett Camille M, Martin Anna, Jyonouchi Harumi, Jyonouchi Soma, Anguera Montserrat C, Romberg Neil CD40LG duplication-associated autoimmune disease is silenced by nonrandom X-chromosome inactivation. The Journal of allergy and clinical immunology 141: 2308-2311.e7, 2018.

Syrett Camille M, Sindhava Vishal, Sierra Isabel, Dubin Aimee H, Atchison Michael, Anguera Montserrat C Diversity of Epigenetic Features of the Inactive X-Chromosome in NK Cells, Dendritic Cells, and Macrophages. Frontiers in immunology 9: 3087, 2018.

Syrett Camille M, Sindhava Vishal, Hodawadekar Suchita, Myles Arpita, Liang Guanxiang, Zhang Yue, Nandi Satabdi, Cancro Michael, Atchison Michael, Anguera Montserrat C Loss of Xist RNA from the inactive X during B cell development is restored in a dynamic YY1-dependent two-step process in activated B cells. PLoS genetics 13: e1007050, 2017.

Wang Jianle, Anguera Montserrat C In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells. Journal of visualized experiments : JoVE : , 2017.

Penkala Ian, Wang Jianle, Syrett Camille M, Goetzl Laura, López Carolina B, Anguera Montserrat C lncRHOXF1, a Long Noncoding RNA from the X Chromosome That Suppresses Viral Response Genes during Development of the Early Human Placenta. Molecular and cellular biology 36: 1764-75, 2016.

Wang Jianle, Syrett Camille M, Kramer Marianne C, Basu Arindam, Atchison Michael L, Anguera Montserrat C Unusual maintenance of X chromosome inactivation predisposes female lymphocytes for increased expression from the inactive X. Proceedings of the National Academy of Sciences of the United States of America 113: E2029-38, 2016.

Luo Mengcheng, Zhou Jian, Leu N Adrian, Abreu Carla M, Wang Jianle, Anguera Montserrat C, de Rooij Dirk G, Jasin Maria, Wang P Jeremy Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. PLoS genetics 11: e1004954, 2015.

Lessing D, Anguera MC, Lee JT. X chromosome inactivation and epigenetic responses to cellular reprogramming. Annu Rev Genomics Hum Genet. 14: 85-110, 2013.

Ph.D (Biochemistry, Molecular and Cellular Biology) Cornell University, 2004

B.A (Environmental Chemistry) University of California, San Diego, 1998