New Bolton Center Kennett Square, PA
Emergencies & Appointments:
Ryan Hospital Philadelphia, PA


Professor of Surgery, University of Pennsylvania School of Veterinary Medicine

Diplomate, American College of Veterinary Surgeons

Clinical Specialties: Soft-Tissue Surgery,
Research Areas: Imaging cancer during surgery, Portosystemic shunts, Brachycephalic airway disease, Laryngeal paralysis

Contact Information:
Department of Clinical Studies
Veterinary Hospital
University of Pennsylvania
3900 Delancey Street

Imaging cancer during surgery,
Portosystemic shunts,
Brachycephalic airway disease,
Laryngeal paralysis

Imaging cancer during surgery,
Portosystemic shunts,
Brachycephalic airway disease,
Laryngeal paralysis,

Phillips H, Corrie J, Engel DM, Duffy DJ, Holt DE, Kendall AR, Schmiedt CW, Vetter A, Meren IL, Follette C, Schaeffer DJ, Mayhew PD, Marks SL Clinical findings, diagnostic test results, and treatment outcome in cats with hiatal hernia: 31 cases (1995-2018). Journal of Veterinary Internal Medicine : , 2019.

Tarrant JC, Holt DE, Durham AC Co-occurrence of Nasal Polyps and Neoplasms of the Canine Nasal Cavity. Veterinary Pathology : 300985819854438, 2019.

Mayhew PD, Boston SE, Zwingenberger AL, Giuffrida MA, Runge JJ, Holt DE, Raleigh JS, Singh A, Culp WTN, Case JB, Steffey MA, Balsa IM Perioperative morbidity and mortality in dogs with invasive adrenal neoplasms treated by adrenalectomy and cavotomy. Veterinary Surgery : VS 48: 742-750, 2019.

Morris Katherine P, Singh Ameet, Holt DE, Stefanovski D, Singhal S, Bosco J, Capps M, McCallum M, Runge JJ Hybrid single-port laparoscopic cisterna chyli ablation for the adjunct treatment of chylothorax disease in dogs. Veterinary Surgery : VS 48: O121-O129, 2019.

Predina JD, Runge J, Newton A, Mison M, Xia L, Corbett C, Shin M, Sulyok Lydia F, Durham A, Nie S, Singhal S, Holt D Evaluation of Aminolevulinic Acid-Derived Tumor Fluorescence Yields Disparate Results in Murine and Spontaneous Large Animal Models of Lung Cancer. Scientific Reports 9: 7629, 2019.

Hsueh C, Giuffrida M, Mayhew PD, Case JB, Singh A, Monnet E, Holt DE, Cray M, Curcillo C, Runge JJ Evaluation of pet owner preferences for operative sterilization techniques in female dogs within the veterinary community. Veterinary Surgery : VS 47: O15-O25, 2018.

Armstrong AJ, Hauptman JG, Stanley BJ, Klocke E, Burneko M, Holt DE, Runge JJ, Rubin JA Effect of Prophylactic Calcitriol Administration on Serum Ionized Calcium Concentrations after Parathyroidectomy: 78 Cases (2005-2015). Journal of Veterinary Internal Medicine 32: 99-106, 2018.

Baron J, Giuffrida M, Mayhew PD, Singh A, Case JB, Culp WTN, Holt DE, Mayhew KN, Runge JJ Minimally invasive small intestinal exploration and targeted abdominal organ biopsy with a wound retraction device in 42 cats (2005-2015). Veterinary Surgery : VS 46: 925-932, 2017.

Keating JJ, Runge JJ, Singhal S, Nims S, Venegas O, Durham AC, Swan G, NS, Low PS, Holt DE Intraoperative near-infrared fluorescence imaging targeting folate receptors identifies lung cancer in a large-animal model. Cancer 123: 1051-1060, 2017.

Majeski SA, Steffey MA, Mayhew PD, Hunt GB, Holt DE, Runge JJ, Kass PH, Mellema M Postoperative Respiratory Function and Survival After Pneumonectomy in Dogs and Cats. Veterinary Surgery : VS 45: 775-81, 2016.

Keating JJ, Nims S, Venegas O, Jiang J, Holt DE, Kucharczuk JC, Deshpande C, Singhal S Intraoperative imaging identifies thymoma margins following neoadjuvant chemotherapy. Oncotarget 7: 3059-67, 2016.

Holt D, Parthasarathy AB, Okusanya O, Keating J, Venegas O, Deshpande C, Karakousis G, Madajewski B, Durham A, Nie S, Yodh AG, Singhal S Intraoperative near-infrared fluorescence imaging and spectroscopy identifies residual tumor cells in wounds. Journal of Biomedical Optics 20: 76002, 2015.

Judy RP, Keating JJ, DeJesus EM, Jiang JX, Okusanya OT, Nie S, Holt DE, Arlauckas SP, Low PS, Delikatny EJ, Singhal S Quantification of tumor fluorescence during intraoperative optical cancer imaging. Scientific Reports 5: 16208, 2015.

Rubin JA, Holt DE, Reetz JA, Clarke DL Signalment, clinical presentation, concurrent diseases, and diagnostic findings in 28 dogs with dynamic pharyngeal collapse (2008-2013). Journal of Veterinary Internal Medicine 29: 815-21, 2015.

Guevara JL, Holmes ES, Reetz J, Holt DE Porcupine quill migration in the thoracic cavity of a German shorthaired pointer. Journal of the American Animal Hospital Association 51: 101-6, 2015.

Wormser C, Singhal S, Holt DE, Runge JJ Thoracoscopic-assisted pulmonary surgery for partial and complete lung lobectomy in dogs and cats: 11 cases (2008-2013). Journal of the American Veterinary Medical Association 245: 1036-41, 2014.

Holt DE, Henthorn P, Howell VM, Robinson BG, Benn DE Succinate dehydrogenase subunit D and succinate dehydrogenase subunit B mutation analysis in canine phaeochromocytoma and paraganglioma. Journal of Comparative Pathology 151: 25-34, 2014.

Lo AJ, Holt DE, Brown DC, Schlicksup MD, Orsher RJ, Agnello KA Treatment of aldosterone-secreting adrenocortical tumors in cats by unilateral adrenalectomy: 10 cases (2002-2012). Journal of Veterinary Internal Medicine 28: 137-43, 2014.

Okusanya OT, Holt D, Heitjan D, Deshpande C, Venegas O, Jiang J, Judy R, DeJesus E, Madajewski B, Oh K, Wang M, Albelda SM, Nie S, Singhal S Intraoperative near-infrared imaging can identify pulmonary nodules. The Annals of Thoracic Surgery 98: 1223-30, 2014.

Holt D, Okusanya O, Judy R, Venegas O, Jiang J, DeJesus E, Eruslanov E, Quatromoni J, Bhojnagarwala P, Deshpande C, Albelda S, Nie S, Singhal S Intraoperative near-infrared fluorescence imaging and spectroscopy identifies residual tumor cells in wounds. PloS One 9: e103342, 2014.

Schlicksup MD, Holt DE, Mai W, Holmes ES, Agnello KA The effect of abaxial retraction on pelvic geometry after pelvic symphysiotomy. Veterinary Surgery : VS 42: 958-62, 2013.

Runge JJ, Curcillo PG, King SA, Podolsky ER, Holt DE, Davidson J, Agnello KA Initial application of reduced port surgery using the single port access technique for laparoscopic canine ovariectomy. Veterinary Surgery : VS 41: 803-6, 2012.

Shanaman M, Seiler G, Holt DE Prevalence of clinical and subclinical middle ear disease in cats undergoing computed tomographic scans of the head. Veterinary Radiology & Ultrasound : The Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association 53: 76-9, 2012.

Clarke DL, Holt DE, King LG Partial resolution of hypoplastic trachea in six english bulldog puppies with bronchopneumonia. Journal of the American Animal Hospital Association 47: 329-35, 2011.

Holt DE, Runge J Use of skin stretchers to elongate a peninsular thoracodorsal axial pattern flap for antebrachial wound closure in a dog. Veterinary Surgery : VS 40: 352-6, 2011.

Holt DE, Goldschmidt MH Nasal polyps in dogs: five cases (2005 to 2011). The Journal of Small Animal Practice 52: 660-3, 2011.

Busschers E, Epstein KL, Holt DE, Parente EJ Extraluminal, C shaped polyethylene prostheses in two ponies with tracheal collapse. Veterinary Surgery : VS 39: 776-83, 2010.

Holt DE, Mover MR, Brown DC Serologic prevalence of antibodies against canine influenza virus (H3N8) in dogs in a metropolitan animal shelter. Journal of the American Veterinary Medical Association 237: 71-3, 2010.

Eisele J, McClaran JK, Runge JJ, Holt DE, Culp WT, Liu S, Long F, Bergman PJ Evaluation of risk factors for morbidity and mortality after pylorectomy and gastroduodenostomy in dogs. Veterinary Surgery : VS 39: 261-7, 2010.

BVSc (Veterinary Science) University of Sydney, Sydney, Australia, 1983