Located in the Rosenthal Building at Penn's School of Veterinary Medicine (Penn Vet), the Extracellular Vesicle (EV) Core Facility provides comprehensive or selected services in the necessary isolation, quantification and characterization of EVs.
Isolation of EV is based on size exclusion using high-performance (SEC-HPLC) or gravity fed (e.g. iZon column) liquid chromatography, ultracentrifugation, and/or density gradient ultracentrifugation. We can accurately characterize EV particle size distribution and concentration using resistive pulse sensing techniques (nCS1, Spectradyne, LLC) and Nanoparticle tracking analysis. Immunophenotype can be accomplished using nanoscale flow cytometry and/or chip array (ExoViewTM) techniques.
Additionally, we provide services in training and education for individuals and lab groups in all methods above and study design consultation to ensure that your EV work is of the highest quality and prepared for high impact publication in this exciting and rapidly growing field.