Located in the Rosenthal Building at Penn's School of Veterinary Medicine (Penn Vet), the Extracellular Vesicle (EV) Core Facility provides comprehensive or selected services in the necessary isolation, quantification and characterization of EVs.
Isolation of EV is based on size exclusion using high-performance liquid chromatography (SEC-HPLC; Agilent) or gravity fed columns (coupled with an automatic fraction collector (Izon), ultracentrifugation, and/or density gradient ultracentrifugation. We can accurately characterize EV particle size distribution and concentration using resistive pulse sensing techniques (nCS1, Spectradyne, LLC) and/or Nanoparticle tracking analysis. Immunophenotype can be accomplished using nanoscale flow cytometry (NanoFCM) and/or chip array (ExoViewTM) techniques (Unchained Labs).
Additionally, we provide services in training and education for individuals and lab groups in all methods above and study design consultation to ensure that your EV work is of the highest quality and prepared for high impact publication in this exciting and rapidly growing field.