Frequently Asked Questions

Requests are prioritized on a ‘first-come, first-serve’ basis. Upon receipt of your request, someone will respond to you within the week to further discuss your project. We try our best to schedule your request within 2 weeks.

CMice are transferred to Investigators around weaning age (3-4 weeks).  The average turn around time from receipt of request to transfer of pups is bout 8-10 weeks. If genotyping is necessary, tail samples will be provided to the requesting lab prior to weaning age. All genotyping must be completed before pups are weaned. Labs will be charged daily cage rates for any pups remaining in the Transgenic Core vivarium after weaning age unless special arrangements have been made.

• Injections are done in a mixed background (C57BL/6J x B6D2F1/J)
• Injection into pure C57BL/6J background available upon request
• Additional Fees will be charged if requesting use of alternate strain not available in house

After microinjection, embryos are implanted into the oviduct or uterine horn of pseudo-pregnant Swiss Webster Females and allowed to grow to term. Swiss females are known for being wonderful mothers and their ability to carry large litters to term.

We recommend mating your chimeras to C57BL6 females so you can use coat color to help determine possible germline transmission. Since we use ES Cells derived from agouti mice, mating your chimeras to Bl6 females would result in easy to identify agouti pups for genotyping. Please note, if the ES cells are heterozygous then only 50% of the agouti pups will be transgenic.