Generating Transgenic Mice
Pronuclear DNA & CRISPR Injections
Direct microinjection into the pronuclei of fertilized single-cell mouse embryos
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- Cas9 protein provided and final injection mixture done in house (unless otherwise specified)
- Injections done in mixed B6/B6D2 Background, but we also offer pure B6 Background upon request (injection into any other background will incur extra fees)
- Tail samples will be provided for genotyping by investigators
- Desired pups will be transferred at weaning age (21 days)
Embryonic Stem Cell (ES-cell) Derivation and Injections
Injection of Embryonic Stem Cells in blastocysts (Day 3.5 embryos).
Intra-cytoplasmic Sperm Injection (ICSI)
Injection of a single sperm into cytoplasm of oocyte.
In Vitro Fertilization
Fertilization of mature oocytes with capacitated sperm in a culture dish.
Cryopreservation is a cost effective way to preserve important mouse lines without the costs of keeping live mice. It also allows for ease of sharing mouse strains with collaborators across the world.