New Bolton Center Kennett Square, PA
Emergencies & Appointments:
Ryan Hospital Philadelphia, PA
Transgenic Mouse Core Services

Our Services

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Generating Transgenic Mice

Pronuclear DNA & CRISPR Injections Direct microinjection into the pronuclei of fertilized single-cell mouse embryos

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    • Cas9 protein provided and final injection mixture done in house (unless otherwise specified)
    • Injections done in mixed B6/B6D2 Background, but we also   offer pure B6 Background upon request (injection into any other background will incur extra fees)
    • Tail samples will be provided for genotyping by investigators
    • Desired pups will be transferred at weaning age (21 days)

Embryonic Stem Cell (ES-cell) Derivation and Injections

Injection of Embryonic Stem Cells in blastocysts (Day 3.5 embryos).

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    • Chimeras will be determinable by coat color
    • Chimeras will be transferred at weaning age (21 days)
    • The cost of your injection includes 2 clones from one target
    • We inject an average of 30 embryos per clone

    For Es Cell derivation & gene targeting please contact Chris Lengner ( & Stephanie Adams (

    We offer a 20% discount on your Injections if you choose to utilize our in house Es Cell derivation services.

Intra-cytoplasmic Sperm Injection (ICSI)

Injection of a single sperm into cytoplasm of oocyte.

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    • Recommended for rescue of mouse lines
    • Requesting lab must provided sperm sample

    We do not charge you a service fee unless we produce a founder.

In Vitro Fertilization

Fertilization of mature oocytes with capacitated sperm in a culture dish.

Sperm/embryo Cryopreservation

Cryopreservation is a cost effective way to preserve important mouse lines without the costs of keeping live mice. It also allows for ease of sharing mouse strains with collaborators across the world.

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    For sperm cryopreservation we require 2 males per strain. From those 2 males we can freeze down 20 sperm straws (10 straws per male). We Recommend males be no younger than 12 weeks before freezing. If freezing down current breeding males, please remove from breeding cage 1 week prior to freezing.

    For embryo freezing, we recommend transferring 10 females 6-8 weeks old.

    Their will be a $25/yr storage fee for anyone wanting to store their sperm straws in house.