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Through evanescent wave illumination that can be adjusted to penetrate as little as 70 nm, events at or near the cell membrane can be imaged with high Z-Axis resolution.
This microscope is available by special arrangement with the laboratory of Dr. Bruce Freedman. Please contact Dr. Freedman or Gordon Ruthel, the core manager, directly to schedule use of the TIRF microscope.
Registered users can access the PVIC online scheduler to reserve and use the Penn Vet Imaging Core instruments.
If you are not already registered, please contact the core manager.
This movie shows a TIRF microscopy timelapse series of GFP-tagged ebola VP40 protein (green) produced by a transfected HEK293T cell. Images were taken on a Leica TIRF microscope with a 100x oil immersion lens. Evanescent wave excitation from a 488 nm laser was set to a penetration depth of 70 nm, such that only events at or near the cell/coverglass interface were visualized. The TIRF microscope is available to Penn Vet Imaging Core users by special arrangement with Dr. Bruce Freedman.
Movie courtesy of Jonathan Madara, Gordon Ruthel, Ron Harty, and Bruce Freedman, Department of Pathobiology, UPenn School of Veterinary Medicine.