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    What We do

    The Penn Vet Imaging Core (PVIC) provides access to cutting-edge optical imaging capabilities for researchers at the University of Pennsylvania, Children’s Hospital of Philadelphia, and Wistar Institute.

    The PVIC includes instruments to perform widefield, confocal, multiphoton, fluorescence lifetime, and total internal reflection fluorescence (TIRF) microscopy, as well as software tools for image analysis. 

    The Core’s highly experienced staff is focused on making this technology accessible to all labs, regardless of prior level of experience.

    About Us

    Discover the cutting-edge imaging instruments at our facility, meticulously designed to cater to a wide array of research needs. Elevate your experiments with our equipment, tailored to boost your capabilities. Explore our tools for invaluable resources that support your work. Our expert team is always on hand to ensure you maximize the benefits of our services. Dive into our extensive publications that highlight the groundbreaking research achieved with our state-of-the-art facilities.

    Instruments & Applications

    The Penn Vet Imaging Core (PVIC) has a variety of microscopes available to meet a broad range of imaging needs. Information on the specific instruments available in the PVIC is provided below, along with examples of some of the types of work that can be done with each of these microscopes.

    Our core requires many different tools related to our service. Please review what we recommend utilizing to get the most out of your research.

    Publications

    Role of nuclear localization in the regulation and function of T-bet and Eomes in exhausted CD8 T cells. McLane LM, Ngiow SF, Chen Z, Attanasio J, Manne S, Ruthel G, Wu JE, Staupe RP, Xu W, Amaravadi RK, Xu X, Karakousis GC, Mitchell TC, Schuchter LM, Huang AC, Freedman BD, Betts MR, Wherry. EJ. Cell Rep. 2021 May 11;35(6):109120.

    Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening. Tichy ED, Ma N, Sidibe D, Loro E, Kocan J, Chen DZ, Khurana TS, Hasty P, Mourkioti. F. Cell Rep. 2021 May 11;35(6):109098.

    LINGO3 regulates mucosal tissue regeneration and promotes TFF2 dependent recovery from colitis. Zullo KM, Douglas B, Maloney NM, Ji Y, Wei Y, Herbine K, Cohen R, Pastore C, Cramer Z, Wang X, Wei W, Somsouk M, Hung LY, Lengner C, Kohanski MH, Cohen NA, Herbert DR. Scand. J Gastroenterol. 2021 May 3:1-15. Online ahead of print.

    IL-33 promotes innate lymphoid cell-dependent IFN-γ production required for innate immunity to Toxoplasma gondii. Clark JT, Christian DA, Gullicksrud JA, Perry JA, Park J, Jacquet M, Tarrant JC, Radaelli E, Silver J, Hunter CA. Elife. 2021 Apr 30;10:e65614.

    Collagen molecular phenotypic switch between non-neoplastic and neoplastic canine mammary tissues. Terajima M, Taga Y, Brisson BK, Durham AC, Sato K, Uzawa K, Saito T, Hattori S, Sørenmo KU, Yamauchi M, Volk SW. Sci Rep. 2021 Apr 21;11(1):8659.

    Regulation of extracellular matrix assembly and structure by hybrid M1/M2 macrophages. Witherel CE, Sao K, Brisson BK, Han B, Volk SW, Petrie RJ, Han L, Spiller KL. Biomaterials. 2021 Feb;269:120667.

    Dll1+ quiescent tumor stem cells drive chemoresistance in breast cancer through NF-κB survival pathway. Kumar S, Nandi A, Singh S, Regulapati R, Li N, Tobias JW, Siebel CW, Blanco MA, Klein-Szanto AJ, Lengner C, Welm AL, Kang Y, Chakrabarti. R. Nat Commun. 2021 Jan 18;12(1):432.

    Angiomotin Counteracts the Negative Regulatory Effect of Host WWOX on Viral PPxY-Mediated Egress. Liang J, Ruthel G, Sagum CA, Bedford MT, Sidhu SS, Sudol M, Jaladanki CK, Fan H, Freedman BD, Harty RN. 2021. J Virol. 95:e00121-21. Online ahead of print.

    Distinct effects of different matrix proteoglycans on collagen fibrillogenesis and cell-mediated collagen reorganization. Chen D, Smith LR, Khandekar G, Patel P, Yu CK, Zhang K, Chen CS, Han L, Wells RG. Sci Rep. 2020 Nov 4;10(1):19065.

    Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells. Hwang YS, Suzuki S, Seita Y, Ito J, Sakata Y, Aso H, Sato K, Hermann BP, Sasaki K. Nat Commun. 2020 Nov 9;11(1):5656.

    Loss of ELF5-FBXW7 stabilizes IFNGR1 to promote the growth and metastasis of triple-negative breast cancer through interferon-γ signalling. Singh S, Kumar S, Srivastava RK, Nandi A, Thacker G, Murali H, Kim S, Baldeon M, Tobias J, Blanco MA, Saffie R, Zaidi MR, Sinha S, Busino L, Fuchs SY, Chakrabarti R. Nat Cell Biol. 2020 May;22(5):591-602. Epub 2020 Apr 13.

    Autophagy mitigates ethanol-induced mitochondrial dysfunction and oxidative stress in esophageal keratinocytes. Chandramouleeswaran PM, Guha M, Shimonosono M, Whelan KA, Maekawa H, Sachdeva UM, Ruthel G, Mukherjee S, Engel N, Gonzalez MV, Garifallou J, Ohashi S, Klein-Szanto AJ, Mesaros CA, Blair IA, Pellegrino da Silva R, Hakonarson H, Noguchi E, Baur JA, Nakagawa H. PLoS One. 2020 Sep 23;15(9):e0239625.

    Hemostasis vs. homeostasis: Platelets are essential for preserving vascular barrier function in the absence of injury or inflammation. Gupta S, Konradt C, Corken A, Ware J, Nieswandt B, Di Paola J, Yu M, Wang D, Nieman MT, Whiteheart SW, Brass LF. Proc Natl Acad Sci USA. 2020 Sep 14:202007642.

    Critical Decrease in the Level of Axon Guidance Receptor ROBO1 in Rod Synaptic Terminals Is Followed by Axon Retraction. Appelbaum T, Santana E, Aguirre GD. Invest Ophthalmol Vis Sci. 2020 Mar 9;61(3):11.

    Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury. Khandekar G, Llewellyn J, Kriegermeier A, Waisbourd-Zinman O, Johnson N, Du Y, Giwa R, Liu X, Kisseleva T, Russo PA, Theise ND, Wells RG. J Hepatol. 2020 Jan;72(1):135-145.

    Intratumoral collagen signatures predict clinical outcomes in feline mammary carcinoma. Rosen S, Brisson BK, Durham AC, Munroe CM, McNeill CJ, Stefanovski D, Sørenmo KU, Volk SW. PLoS One. 2020 Aug 10;15(8):e0236516.

    Alcohol-induced CYP2E1, mitochondrial dynamics and retrograde signaling in human hepatic 3D organoids.Angireddy R, Chowdhury AR, Zielonka J, Ruthel G, Kalyanaraman B, Avadhani NG. Free Radic Biol Med. 2020 Jul 29;159:1-14.

    Angiomotin regulates budding and spread of Ebola virus. Han Z, Ruthel G, Dash S, Berry CT, Freedman BD, Harty RN, Shtanko O. J Biol Chem. 2020 Jun 19;295(25):8596-8601.

    SKP1 drives the prophase I to metaphase I transition during male meiosis. Guan Y, Leu NA, Ma J, Chmátal L, Ruthel G, Bloom JC, Lampson MA, Schimenti JC, Luo M, Wang P. J. Sci Adv. 2020 Mar 25;6(13):eaaz2129.

    Modular mimicry and engagement of the Hippo pathway by Marburg virus VP40: Implications for filovirus biology and budding. Han Z, Dash S, Sagum CA, Ruthel G, Jaladanki CK, Berry CT, Schwoerer MP, Harty NM, Freedman BD, Bedford MT, Fan H, Sidhu SS, Sudol M, Shtanko O, Harty RN. PLoS Pathog. 2020 Jan 6;16(1):e1008231.

    Spatial mapping of polymicrobial communities reveals a precise biogeography associated with human dental caries. Kim D, Barraza JP, Arthur RA, Hara A, Lewis K, Liu Y, Scisci EL, Hajishengallis E, Whiteley M, Koo H. Proc Natl Acad Sci USA. 2020 Jun 2;117(22):12375-12386.

    Service Fees and Training Opportunities

    The Department of Pathobiology maintains this facility and oversees the fee structure for this service center.

    Find Us

    University of Pennsylvania

    School of Veterinary Medicine
    3800 Spruce Street

    Philadelphia, PA 19104-4539


    Our Team

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    Gordon Ruthel, PhD