Contact
New Bolton Center Kennett Square, PA
Emergencies & Appointments:
610-444-5800
Directions
Ryan Hospital Philadelphia, PA
Emergencies:
215-746-8911
Appointments:
215-746-8387
Directions

Leica DM6000B Upright Widefield Fluorescence Microscope


Description

This upright widefield microscope is equipped with both color and monochrome cameras, making it suitable for imaging either histology slides or fluorescently labeled samples.


Applications

  • Acquisition of full-color images from histology slides
  • Acquisition of fluorescence images (up to 4 colors)
  • Automated tile and stitch acquisition of large areas
  • Motorized Z-stack acquisition for deconvolution with Huygens software
  • Differential interference contrast images with 40x, 63x, and 100x

Leica DM6000B Upright Widefield

DM6000B microscope, Imaging Core, Penn Vet
Technical Specifications: Leica DM6000B Widefield tech sheet (PDF)

Scheduling Core Instruments

Registered users can access the PVIC online scheduler to reserve and use the Penn Vet Imaging Core instruments.

If you are not already registered, please contact the core manager.

Application Examples

Fluorescence microscopy

Leishmania, E600, Imaging Core Leishmania2, E600, Imaging Core

The parasite Leishmania major (promastigote stage; red) is shown here among alternatively activated (M2) macrophages (green) and resident peritoneal macrophages (blue).

These images were taken on the Penn Vet Imaging Core’s Nikon E600 fluorescence microscope with a 100x oil immersion lens.

Images are courtesy of Tiffany Weinkopff and Phillip Scott, Dept. of Pathobiology, UPenn School of Veterinary Medicine.


Transmitted light microscopy

Nikon E600 hair follicles

Cross sections of a primary (larger) and multiple secondary (smaller) hair follicles characteristic of compound hair follicles in dogs and cats.  This image was taken on the Penn Vet Imaging Core’s Nikon E600 microscope with a 10x objective lens.

Sample prepared by the Penn Vet Diagnostic Lab, photo by Gordon Ruthel, description provided by Charles Bradley.


Transmitted light microscopy

Nikon E600 keratin-filled cyst

A keratin-filled cyst at the periphery of an infundibular keratinizing acanthoma, a common benign follicular neoplasm in dogs.  This image was taken on the Penn Vet Imaging Core’s Nikon E600 microscope with a 20x objective lens. 

Sample prepared by the Penn Vet Diagnostic Lab, photo by Gordon Ruthel, description provided by Charles Bradley.


Transmitted light microscopy

Nikon E600 Erythrocytes Dog Brain

Erythrocytes within a meningeal arteriole of a dog brain. This image was taken on the Penn Vet Imaging Core’s Nikon E600 microscope with a 40x objective lens and DIC optics. 

Sample prepared by the Penn Vet Diagnostic Lab, photo by Gordon Ruthel, description provided by Charles Bradley.