Penn Cytomics is the sole flow cytometry shared resource laboratory (SRL) at the University of Pennsylvania. Our facility has 35+ instruments, which include analyzers, cell sorters, and small particle detectors. We also have a dual-fluorescence cell counter/viability instrument and a tissue dissociator for cell preparation. We provide both on-site and off-site support to our instrument users, including analyzer and cell sorter training. We also have a research and development team that collaborates/consults with principal investigators in developing high-dimensional panels, as well as staining, acquisition, and analysis. Our core has ~10 nearby satellite locations to accommodate user needs. Penn Cytomics is an Abramson Cancer Center supported facility and has been recognized as "exceptional" by the National Cancer Center for over 30 years.